Background and Aim: Mitochondrial dynamics is affected by division and fusion, and exercise training may have an effect on it. The purpose of this research is to determine the effect of eight weeks of moderate intensity interval training on the gene expression of indicators involved in the division and integration of mitochondria in the Soleus muscle of rats. Materials and Methods: For this purpose, 12 male Wistar rats were randomly divided into two equal groups (Six rats in each group) of control and moderate intensity interval training (MIT). First, the residual test and the average maximum speed of the rats were calculated to design the training program. The exercise program included running on the treadmill with an intensity of 60-65% of the maximum speed (six intervals of four minutes) and active rest with an intensity of 30% of the maximum speed (six intervals of two minutes), In compliance with the principle of overload which was carried out for 8 weeks (5 sessions per week). In order to evaluate the gene expression changes of PGC-1α, Opa1, Fis1, Drp1, Mfn1/2 indices, the plantar muscle tissue of rats was extracted. The expression level of research genes was measured using pcr method. The data were analyzed using independent t-statistics at the significance level (p≤0.05). Results: The results showed that exercise in the mit group significantly decreased the expression of Drp1 and Fis1 genes and increased the expression of PGC-1α, Mfn1 and Mfn2 genes. But MIT exercises did not significantly change the expression of Opa1 gene. Conclusion: According to the findings of the present study, it can be said that the adaptation of intermittent exercise has a protective effect on the dynamics of mitochondrial quality, but in fact, it has not completely prevented the occurrence of mitochondrial division.