Document Type : Original Article
Authors
1 PhD student in exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
2 Associate professor of exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
3 Associate professor of physiology, Department of biology, Shiraz branch, Islamic Azad University, Shiraz, Iran
4 Assistant professor of exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
Abstract
Background and Aim: Dysregulated miRNAs play critical roles in many disorders such as pain in Parkinson's disease. The aim of this study was to examine the effect of swimming training on hypocampic miRNAs gene expression related to pain in rats with Parkinson's disease (PD). Material and Methods: Twenty-one male Wistar rats (age eight to ten weeks) were divided into healthy control (n=7), PD (n=7) and training (n=7) group. PD induced by injection of 1 mg/kg reserpine to rats in PD and training groups. The rats in the training group performed 6 weeks high-intensity interval training, including 20 times of 30 seconds of swimming with 30 seconds of rest between each time and 3 times a week. mir-23b and mir-let-7 gene expressions were measured in hippocampus using Real Time-PCR. Data were analyzed using one-way ANOVA and LSD post hoc test were run using SPSS-22 at the p<0.05. Results: Data indicated that mir-23b gene expression was lower in PD group compare to the healthy control group (p=0.01), while no significant difference was observed between PD group and training group (p=0.22) and between healthy control group and training group (p=0.09). Data revealed that mir-let-7 gene expression was lower in the PD group compare to the healthy control group, but it not achieved significant statistically (p=0.50). Also, no significant difference was observed between PD group and training group (p=0.82) and between healthy control group and training group (p=0.64). Conclusion: In summary it seems that high-intensity interval swimming training did not affect miRNAs related pain in rats with PD; however future studies are needed.
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