Document Type : Original Article
Authors
1 MSc of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Payame Noor University, Alborz, Iran.
2 Assistant Professor at Exercise Physiology Department, Faculty of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.
Abstract
Background and Aim: The development of new blood vessels involves the stimulation of growth, migration, and stabilization, which is influenced by various stimulating and inhibiting factors such as Thrombospondin-2 (TSP-2) and transforming growth factor-beta (TGF-β). This study aimed to compare the effect of eight weeks of different aerobic training methods on the expression of TSP-2 and TGF-β genes in subcutaneous adipose tissue of rats. Materials and Methods: The study conducted an experimental research on 32 male Wistar rats to investigate the effects of different training protocols on gene expression. The rats were divided into four groups (n=8): scheme, high-intensity training (HIT), moderate-intensity training (MIT), and high-intensity interval training (HIIT). The training protocols involved treadmill running for eight weeks, with varying intensities and durations for each group. Gene expression was measured by RT-PCR method. The data were analyzed with one-way ANOVA and Tukey’s tests at a significance level of p≤0.05. Results: The results of the study show that all three training methods led to a significant reduction in TGF-β expression compared to the scheme group. However, no significant difference was observed between training methods. It was also found that only the HIT and MIT methods caused a significant decrease in TSP-2 expression compared to the scheme group, and that HIT could significantly reduce the expression of TSP-2 compared to the HIIT protocol. Conclusion: In conclusion, it is suggested that aerobic exercises, particularly HIT with specific intensity and duration, may be effective in improving blood supply to fat tissue and subsequently reducing fat mass through their impact on the expression of angiostatic genes.
Keywords
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