Document Type : Original Article

Authors

1 MSc in Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran.

2 Associate Professor, Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, Babolsar, Iran.

3 Assistant professor, Department of Developmental Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.

Abstract

Background and Aim: Previous studies have shown that regular aerobic exercise improves fertility. However, there were conflicting results regarding the effect of resistance training on fertility. The aim of this study was to investigate the effects of resistance training (RT) with two different volumes on some semen parameters and serum levels of sex hormones in male rats. Materials and Methods: Twenty-four male Wistar rats (157.7±7.6 gr) were randomly divided into 3 groups; Control, High Volume RT, Low volume RT (8 rats in each group). The resistance training program included climbing the ladder for 8 weeks (3 days/week, every other day). Body weight, serum concentrations of testosterone (Ts), Follicle Stimulating Hormone (FSH) and luteinizing hormone (LH) were measured as well as the number and total sperm motility was calculated. Data were analyzed using one-way ANOVA and Bonferroni post hoc tests at the significant level of pResults: After eight weeks of resistance training serum levels of LH in High volume RT group were lower compared with control group (p=0.03). Higher levels of FSH in Low volume RT group was found compared with High volume RT group (p=0.009). There were no significant differences between groups in serum levels of testosterone, body weight values, number and total sperm motility) P>0.05). Conclusion: According to these results, it seems that a disproportionate increase in resistance training volume can lead to decrease the levels of some sex hormones. Therefore, it may lead to disruption of the spermatogenesis.

Keywords

Ahmadi, J., Hassani, A., & Donyai, A. (2015). The effect of ginseng supplementation and six weeks of resistance training on aerobic and anaerobic power in sedentary male students. Journal of Practical Studies of Biosciences in Sport, 3(5), 45-55. [Persian]
Ahtiainen, J. P., Nyman, K., Huhtaniemi, I., Parviainen, T., Helste, M., Rannikko, A., … & Häkkinen, K. (2015). Effects of resistance training on testosterone metabolism in younger and older men. Experimental Gerontology, 69, 148-158.
Alessio, H. M. (1993). Exercise-induced oxidative stress. Medicine and Science in Sports and Exercise, 25(2), 218-224.
Barari, A., Ayatollahi, A., Shirali, S., Ghasemi, M., Hosseini, A., Ekrami, A., & Khandan Del, A. (2014). Effect of endurance and resistance training on parameters related to sexual function in men. Medical Laboratory Journal, 8(3), 47-53. [Persian]
Bird, S. P., Tarpenning, K. M., & Marino, F. E. (2005). Designing resistance training programmes to enhance muscular fitness. Sports Medicine, 35(10), 841-851.
Clarkson, P. M., & Thompson, H. S. (2000). Antioxidants: what role do they play in physical activity and health? The American Journal of Clinical Nutrition, 72(2), 637s-646.
Fry, A. C., Kraemer, W. J., Stone, M. H., Warren, B. J., Kearney, J. T., Maresh, C. M., … & Fleck, S. J. (1993). Endocrine and performance responses to high volume training and amino acid supplementation in elite junior weightlifters. International Journal of Sport Nutrition and Exercise Metabolism, 3(3), 306-322.
Ghanbari-Niaki, A. (2006). Ghrelin and glucoregulatory hormone responses to a single circuit resistance exercise in male college students. Clinical Biochemistry, 39(10), 966-970.
Gill, J. M., & Cooper, A. R. (2008). Physical activity and prevention of type 2 diabetes mellitus. Sports Medicine, 38(10), 807-824.
Gotshalk, L. A., Loebel, C. C., Nindl, B. C., Putukian, M., Sebastianelli, W. J., Newton, R. U., … & Kraemer, W. J. (1997). Hormonal responses of multiset versus single-set heavy-resistance exercise protocols. Canadian Journal of Applied Physiology, 22(3), 244-255.
Grandys, M., Majerczak, J., Zapart-Bukowska, J., Kulpa, J., & Zoladz, J. A. (2011). Gonadal hormone status in highly trained sprinters and in untrained men. The Journal of Strength & Conditioning Research, 25(4), 1079-1084.
Hackney, A. C. (2008). Effects of endurance exercise on the reproductive system of men: the “exercise-hypogonadal male condition”. Journal of Endocrinological Investigation, 31(10), 932-938.
Holten, M. K., Zacho, M., Gaster, M., Juel, C., Wojtaszewski, J. F., & Dela, F. (2004). Strength training increases insulin-mediated glucose uptake, GLUT4 content, and insulin signaling in skeletal muscle in patients with type 2 diabetes. Diabetes, 53(2), 294-305.
Howlett, K. F., Sakamoto, K., Garnham, A., Cameron-Smith, D., & Hargreaves, M. (2007). Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle. Diabetes, 56(6), 1608-1614.
Irvine, D. S. (1996). Glutathione as a treatment for male infertility. Reviews of Reproduction, 1(1), 6-12.
Jarjisian, S. G., Piekarski, D. J., Place, N. J., Driscoll, J. R., Paxton, E. G., Kriegsfeld, L. J., & Zucker, I. (2013). Dorsomedial hypothalamic lesions block Syrian hamster testicular regression in short day lengths without diminishing increased testosterone negative-feedback sensitivity. Biology of Reproduction, 89(2), 1-23.
Jurca, R., Lamonte, M. J., Barlow, C. E., Kampert, J. B., Church, T. S., & Blair, S. N. (2005). Association of muscular strength with incidence of metabolic syndrome in men. Medicine and Science in Sports and Exercise, 37(11), 1849.
Kageyama, K., Kumata, Y., Akimoto, K., Takayasu, S., Tamasawa, N., & Suda, T. (2011). Ghrelin stimulates corticotropin-releasing factor and vasopressin gene expression in rat hypothalamic 4B cells. Stress, 14(5), 520-529.
Kilian, Y., Engel, F., Wahl, P., Achtzehn, S., Sperlich, B., & Mester, J. (2016). Markers of biological stress in response to a single session of high-intensity interval training and high-volume training in young athletes. European Journal of Applied Physiology, 116(11-12), 2177-2186.
Kuoppasalmi, K., & Adlercreutz, H. (1985). Interaction between catabolic and anabolic steroid hormones in muscular exercise. Exercise Endocrinology, 65-98.
Leite, R. D., Prestes, J., Rosa, C., De Salles, B. F., Maior, A., Miranda, H., & Simao, R. (2011). Acute effect of resistance training volume on hormonal responses in trained men. Journal of Sports Medicine and Physical Fitness, 51(2), 322.
Meeusen, R., Nederhof, E., Buyse, L., Roelands, B., De Schutter, G., & Piacentini, M. F. (2010). Diagnosing overtraining in athletes using the two-bout exercise protocol. British Journal of Sports Medicine, 44(9), 642-648.
Minetto, M., Lanfranco, F., Baldi, M., Termine, A., Kuipers, H., Ghigo, E., & Rainoldi, A. (2007). Corticotroph axis sensitivity after exercise: comparison between elite athletes and sedentary subjects. Journal of Endocrinological Investigation, 30(3), 215-223.
Padron, O. F., Brackett, N. L., Sharma, R. K., Lynne, C. M., Thomas, A. J., & Agarwal, A. (1997). Seminal reactive oxygen species and sperm motility and morphology in men with spinal cord injury. Fertility and Sterility, 67(6), 1115-1120.
Parastesh, M., Heidarianpour, A., Bayat, M., & Saremi, A. (2016). Effects of Resistance Training on Serum Level of Reproductive Hormones and Sperm Parameters in Type 2 Diabetes Rats. Journal Arak Medical University, 19(8), 26-36. [Persian]
Pollock, M. L., Carroll, J. F., Graves, J. E., Leggett, S. H., Braith, R. W., Limacher, M., & Hagberg, J. M. (1991). Injuries and adherence to walk/jog and resistance training programs in the elderly. Medicine and Science in Sports and Exercise, 23(10), 1194-1200.
Ronkainen, H. (1985). Depressed follicle-stimulating hormone, luteinizing hormone, and prolactin responses to the luteinizing hormone-releasing hormone, thyrotropin-releasing hormone, and metoclopramide test in endurance runners in the hard-training season. Fertility and Sterility, 44(6), 755-759.
Safarinejad, M. R., Azma, K., & Kolahi, A. A. (2009). The effects of intensive, long-term treadmill running on reproductive hormones, hypothalamus–pituitary–testis axis, and semen quality: a randomized controlled study. Journal of Endocrinology, 200(3), 259-271. [Persian]
Saleh, R. A., & Agarwal, A. (2002). Oxidative stress and male infertility: from research bench to clinical practice. Journal of Andrology, 23(6), 737-752.
Samavati Sharif, M. A., Afshar, A., Siavoshy, H., & Keshvary, M. (2017). The effect of two exercises training on some of immune system markers in adolescent athletes. Journal of Practical Studies of Biosciences in Sport, 4(8), 55-65. [Persian]
Sullivan, D. H., Roberson, P. K., Johnson, L. E., Bishara, O., Evans, W. J., Smith, E. S., & Price, J. A. (2005). Effects of muscle strength training and testosterone in frail elderly males. Medicine and Science in Sports and Exercise, 37(10), 1664.
Tsai, K., Hsu, T. G., Hsu, K. M., Cheng, H., Liu, T. Y., Hsu, C. F., & Kong, C. W. (2001). Oxidative DNA damage in human peripheral leukocytes induced by massive aerobic exercise. Free Radical Biology and Medicine, 31(11), 1465-1472.
Vaamonde, D., Da Silva-Grigoletto, M. E., García-Manso, J. M., Vaamonde-Lemos, R., Swanson, R. J., & Oehninger, S. C. (2009). Response of semen parameters to three training modalities. Fertility and Sterility, 92(6), 1941-1946.
Wang, C., Hikim, A. S., Ferrini, M., Bonavera, J. J., Vernet, D., Leung, A., … & Swerdloff, R. S. (2002). Male reproductive ageing: using the brown Norway rat as a model for man. In Endocrine Facets of Ageing: Novartis Foundation Symposium, 242, 82-97. Chichester, UK: John Wiley & Sons, Ltd.
Wheeler, G. D., Wall, S. R., Belcastro, A. N., & Cumming, D. C. (1984). Reduced serum testosterone and prolactin levels in male distance runners. Jama, 252(4), 514-516.
Wu, C. L., Hung, W., Wang, S. Y., & Chang, C. K. (2008). Hormonal responses in heavy training and recovery periods in an elite male weightlifter. Journal of Sports Science & Medicine, 7(4), 560.
Zitzmann, M. (2011). Exercise, Training, and the Hypothalamic–Pituitary–Gonadal Axis in Men. In Hormone Use and Abuse by Athletes, 29, 25-30.