Horieh Karimi; Mahtab Moazami; Ali Yaghoubi; Sadegh Abbasian
Abstract
Background and Aim: Alzheimer's disease is defined as the most common age-related neurodegenerative diseases. Therefore, the aim of the present study was to compare the effects of diets with and without caloric restriction along with continuous training on the expression of selected genes effective in ...
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Background and Aim: Alzheimer's disease is defined as the most common age-related neurodegenerative diseases. Therefore, the aim of the present study was to compare the effects of diets with and without caloric restriction along with continuous training on the expression of selected genes effective in Alzheimer's disease of old male rats. Materials and Methods: For this purpose, 36 old male rats (26 months old, with an average weight of 437.8 ± 41.3 grams) were randomly divided into four groups as control group, continuous training group, continuous training with caloric restriction group, and caloric restriction group. The rats of the training groups performed continuous exercises for eight weeks and 5 sessions per week, by following the overload principle on the treadmill. Also, caloric restriction was initially applied with a 30% reduction and then a 20% reduction in the rats’ food consumed. Continuous exercises were performed for eight weeks and 5 sessions per week (10 minutes to 20 minutes) following the overload principle on a treadmill. Brain tissue was taken 24 hours after the last training session and gene expression was done by real-time PCR method. Furthermore, ANOVA with Tukey's post hoc test was used to analyze the results. Results: The findings demonstrated a significant difference between groups in Tau gene expression (p=0.02). Moreover, there was no significant difference in amyloid beta gene expression and insulin levels in old male rats (p = 0.486). Conclusion: In general, continuous training with and without caloric restriction could not lead to a significant change in APP gene expression. But the results of the research showed significant changes in Tau gene expression in the brain tissue of elderly male rats, preferably with the aim of preventing Alzheimer's.
Zahra Seddighi khovidak; Daruosh Moflehi; Soheil Aminizadeh
Abstract
Background and Aim: A calorie restriction diet is one way of the ways to reduce calorie intake without malnutrition. The purpose of this study was to investigate the effect of eight weeks of high-intensity interval training with calorie restriction of high-fat or standard diet regimens on the expression ...
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Background and Aim: A calorie restriction diet is one way of the ways to reduce calorie intake without malnutrition. The purpose of this study was to investigate the effect of eight weeks of high-intensity interval training with calorie restriction of high-fat or standard diet regimens on the expression of sirtuin 1 (SIRT1), estrogen-related receptor alpha (ERRα) and pyruvate dehydrogenase kinase 4 (PDK4) in the liver of male Wistar rats. Materials and Methods: 48 rats were randomly divided into six groups (n=8): control, high-intensity interval training, high-fat diet with caloric restriction, standard diet with caloric restriction, standard diet with caloric restriction + interval training, and fatty diet with caloric restriction + interval training were divided. High-intensity interval training was performed for 8 weeks and 5 days a week with an intensity of 90 to 100% of the maximum speed in the form of running on a treadmill. Expression of SIRT1, ERRα and PDK4 genes in liver tissue was measured by Real-Time PCR method and one-way ANOVA statistical method was used to compare between groups. Results: Interval training led to a significant increase in ERRα gene expression compared to the control group (p=0.001). Fatty diet with calorie restriction significantly decreased SIRT1 gene expression compared to standard diet with calorie restriction (p=0.002). Also, eight weeks of standard diet with caloric restriction led to a significant increase in PDK4 gene expression compared to the control group (p=0.02). Conclusion: In the condition of calorie restriction, periodic exercise through the up regulation of ERRα gene expression may play a key role in increasing hepatic gluconeogenesis. Interestingly, high-fat diet may have negative effects on the liver even under caloric restriction by reducing the expression of SIRT1, which plays an important role in the antioxidant capacity of the liver.
