The effects of resistance and continuous training on the content of collagen 1 and extracellular matrix (ECM) proteins in the Myocardial tissue of Dexamethasone-induced female rat model

Articles in Press

Document Type : Original Article

Authors

1 Faculty of Physical Education and Sports Sciences, Shahid Chamran University, Ahvaz, Iran

2 Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

10.22077/jpsbs.2025.8212.1912

Abstract

Background and Aim: Glucocorticoids use has been associated with a variety of adverse cardiovascular effects including fluid retention, hypertension, premature atherosclerotic disease, and arrhythmias. The purpose of this study was to investigate the effect of six weeks of resistance and Continuous training on the content of collagen 1 and extracellular matrix proteins in the myocardial tissue of dexamethasone-induced female model rats. Materials and Methods: A total of twelve female rats, aged four weeks were randomly divided into four group: healthy control, DEXA-induced control, resistance training + DEXA, and continuous training + DEXA (n = 3). The resistance training program consisted of three sessions each week for six weeks, during which time the participants would carry a weight and climb a one-meter ladder 14-20times. The continuous exercise program consisted of 16 to 40 minutes of treadmill running at 60-70 ℅ of a rat’s maximum speed, 3days a week for six weeks. Content of type 1 collagen, matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in left ventricle of myocardial tissue after exercise by western blot method and transforming growth factor beta-1 (TGF-β1) were measured by ELISA method. The one-way analysis of variance and Tukey's post hoc test were used to analyze the data at the p≤0.05 level. Results: Dex injection in three groups led to a significant decrease in MMP2 and a significant increase in collagen 1, TIMP1 and TGF-β1 compared to the healthy control group. But, both types of trainings significantly increased MMP2 and significantly decreased collagen type 1, TIMP1 and TGF-β1. Conclusions: It seems that that continuous aerobic and resistance training can simultaneously increase MMP2 activity and decrease COL-I, TGF-β1 and TIMP1 protein content in the left ventricle, a protective effect on the structure and function of the heart ventricle.

Keywords

Journal of Practical Studies of Biosciences in Sport

Articles in Press, Accepted Manuscript
Available Online from 21 January 2025

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