Document Type : Original Article
Authors
1 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.
2 2Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
3 Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
4 , Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
Abstract
Introduction: The aim of this study was investigating the effect of aerobic exercise supplemented with the hydroalcoholic extract of qaraqat fruit on glycemic indices and miR-195 expression in cardiac tissue of rats with type 2 diabetes.
Methods: Male Wistar rats (n=24, mean weight 200±20 grams and eight weeks old) were randomly divided into four groups: 1) diabetic 2) diabetic+supplementation, 3) diabetic+exercise, and 4) diabetic+supplementation+exercise. To induce type 2 diabetes, rats were given a high-fat diet (60%) for two weeks, followed by a fasting streptozocin injection. The rats in the supplemented groups received 250 mg of karaqat fruit extract per kilogram of body weight daily. Aerobic training consisted of treadmill exercise reaching 18-24 m/min for 30-60 minutes during the last week and was performed five days per week for eight weeks. After eight weeks, all rats were anesthetized with a combination of ketamine and xylazine solution. Glucose, insulin, insulin resistance and miR-195 of the cardiac tissue were measured. To analyze the data, a one-way analysis of variance was performed at a significance level of P<0.05.
Results: Fasting blood glucose and insulin resistance decreased significantly after eight weeks of intervention in all groups compared to the diabetic group (P<0.01). In addition, a significant decrease in insulin levels was observed in the groups consuming dietary supplements compared to the diabetic and diabetic+exercise groups. In addition, the expression levels of miR-195 showed a significant decrease in the diabetic+supplement+exercise group compared to the diabetic+exercise group after eight weeks of intervention (P<0.05).
Conclusion: According to the results, aerobic exercise and qaraqat supplementation alone or in combination with each other lead to an improvement in glycemic indices and miR-195 expression. However, supplementation of qaraqat together with aerobic exercise has more favorable effects on the expression of miR-195 in cardiac tissue of rats with type 2 diabetes.
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