Document Type : Original Article
Authors
- Badrkhan Rashwan Ismael 1
- Elaheh Piralaiy 2
- Saeid D. Nikoukheslat 3
- Alireza Rashidpour 4
- Gholamreza Hamidian 5
1 Ph.D student, Exercise physiology, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
2 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.
3 Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
4 Master's Student in Exercise physiology, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
5 Associate Professor in Comparative Histology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Abstract
Background and Aim: Previous research has shown the damaging effects of p53 and miR-34a in type 2 diabetes. However, the effects of exercise training on p53 and miR-34a changes in heart tissue in rats with type 2 diabetes are unclear. Therefore, This study intended to study the effects of aerobic training on the expression genes of p53 and miR-34a in the heart tissue of type 2 diabetic rats. Materials and Methods:For this purpose, 20 male Wistar rats (mean 28±239 g, age 6-8 weeks) were randomly divided into four groups including diabetic control, healthy control, diabetic training, and healthy training. Animal food was provided in the form of free access pellets and water in a 500 ml bottle. To induce type 2 diabetes, a high-fat diet (60%) was performed for two weeks followed by streptozotocin injection in a fasting state. The training protocol comprised eight weeks of running on a treadmill for 30-60 minutes at a speed of 5-10 m/min, five days a week, with the principle of overload. The p53 and miR-34a gene expression in the heart tissue samples was measured by Real-Time PCR. Data were analyzed by one-way ANOVA at the P < 0.05. Results: Eight weeks of T2DM infection resulted in a significant increase in p53 and miR-34a compared to healthy control and healthy training groups (p=0.0001). However, eight weeks of aerobic training showed a significant decrease in miR-34a gene expression (p=0.02) and a non-significant change in p53 gene expression (p=0.05) compared to the diabetic control group. Conclusion: It seems that part of the destructive morphological and cellular effects of type 2 diabetes on heart tissue can be affected through the effects of eight weeks of aerobic training on miR-34a gene expression. However, different training conditions may be needed to see a reduction in p53.
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