Document Type : Original Article
Authors
1 hD Candidate of Sport Science, Islamic Azad University, Bojnourd Branch, Bojnurd, Iran
2 Assistant Professor, Department of Sport Science, Islamic Azad University, Bojnurd Branch, Bojnurd, Iran
3 3Assistant Professor, Department of medical physics and radiology, North Khorasan University of Medical Sciences, Bojnurd, Iran.
4 4Assistant Professor, Department of Advanced Technologies, School of Medicine, Bojnurd, Iran
Abstract
Introduction: The aim of this study was to investigate the effect of four weeks of aerobic training, one and two sessions per day, on motor performance and gene expression of Tropomyosin receptor kinase B (TrkB), and Phosphoinositide 3-kinase (PI3K) tropomyosin kinase B and phosphatidyl-inositol-3-kinase receptors in hippocampal rats with spinal cord injury (SCI).
Methods: This experimental study was conducted on adult and young male Wistar rats. The animals were randomly divided into 6 groups (control group, healthy group with the first exercise protocol, healthy group with the second exercise protocol, SCI group, SCI group with the first exercise protocol, and SCI group with the second exercise protocol; 7 animals in each group). First, animals have been subjected to general anesthesia and SCI. After two weeks for recovery, two types of aerobic exercise programs were performed. After performing the exercises, Basso Beattie Bresnahan (BBB) test and molecular tests were used to measure the expression of the NT4 gene from the hippocampus of the animals, using the qRT-PCR method.
Results: The results showed that four weeks of one- and two-session aerobic exercises caused a significant increase in the gene expression of tropomyosin kinase B receptor and phosphatidyl-inositol-3-kinase compared to the spinal cord injury group. In addition, these training protocols have also played a role in improving movement in animals with spinal cord injury. Therefore, it can be a factor for axonal growth and neuronal survival in spinal cord injury recovery.
Conclusion: The exercise protocols of this study are effective on tropomyosin kinase B and phosphatidyl-inositol-3-kinase receptors gene expression in addition to improving movement in animals with SCI, and can be a factor for axonal growth and neuronal survival in spinal cord injury recovery.
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