Document Type : Original Article
Authors
1 Professor at Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Allameh Tabataba’i University, Tehran, Iran.
2 MS.c in Exercise Physiology, Faculty of Physical Education and Sport Sciences, Allameh Tabataba’i University, Tehran, Iran.
3 Assistant Professor at Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Allameh Tabataba’i University, Tehran, Iran.
4 Ph.D in Exercise Physiology, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University (Tehran Branch).
Abstract
Background and Aim: In wrestling, the rest time between two rounds of the competition is short; therefore, these conditions these conditions cause the body to lose and not completely regenerate its energy reserves, and the person is tired and eventually prone to injury. On the other hand, such activities, which are performed with intense eccentric contractions are associated with mechanical and metabolic disorders. Therefore, the purpose of the present study was to determine the effect of short-term Inflamma-X supplementation on muscle damage and inflammatory markers following the combined SWPT-SWFT protocol in wrestlers. Materials and Methods: 24 young wrestlers were randomly divided into two supplement-exercise groups (12 people) and placebo-exercise groups (12 people). From 10 days before the implementation of the combined protocol, the wrestlers consumed two daily supplements of Inflamma-X with a dose of 15 mg or a placebo with the same amount and number as fasting. Blood samples were taken before the start of the combined protocol, immediately after the first, second and fourth stages of the protocol, and finally 48 hours after the combined protocol. Levels of keratin kinase (CK), lactate dehydrogenase (LDH), reactive protein - C(CRP) indicators were evaluated by the Eliza method. In order to analyze the data, the method of analysis of variance with combined repeated measurement was used at the significance level of p<0.05. Results: In none of the stages of blood sampling, serum CK and LDH values were not significantly different between the two groups (p<0.05). However, the serum CRP values of the supplement group in the fourth and fifth stages were significantly lower than the placebo group (p<0.05); While in the first, second and third blood sampling stages; There was no significant difference between the two groups (p<0.05). Conclusion: It seems that in wrestling competitions, the use of Inflamma-X supplement can be beneficial in the prevention of general inflammation. But more research is needed on the role of this supplement in preventing increased muscle injury markers in wrestlers.
Keywords
Askari, G., Ghiasvand, R., Feizi, A., Ghanadian, S.M., & Karimian, J. (2012). The effect of quercetin supplementation on selected markers of inflammation and oxidative stress. Journal of Research in Medical Sciences: the Oficial Journal of Isfahan University of Medical Sciences, 17(7), 637. [In Persian]. PMCID: PMC3685779.
Bessa, A.L., Oliveira, V.N., Agostini, G.G., Oliveira, R.J., Oliveira, A.C., White, G.E., ... & Espindola, F.S. (2016). Exercise intensity and recovery: biomarkers of injury, inflammation, and oxidative stress. The Journal of Strength & Conditioning Research, 30(2), 311-319. http://dx.doi.org/10.1519/jsc.0b013e31828f1ee9
Blasco, R., Rubio, J., Anguera, A., Ayllón, A., Ramos, D., & Jiménez, J. (2012). Suplementación con bromelina en el daño muscular producido durante el ejercicio físico excéntrico. Estudio Bromesport. Archivos de Medicina Del Deporte, 29(150), 769-783. http://dx.doi.org/10.1016/j.ramd.2014.10.050
Boroumand, N., Samarghandian, S., & Hashemy, S.I. (2018). Immunomodulatory, anti-inflammatory, and antioxidant effects of curcumin. Journal of Herbmed Pharmacology, 7(4), 211-219. [In Persian]. http://dx.doi.org/10.15171/jhp.2018.33
Cheshier, B.C., & Jacobson, B.H. (2021). The effectiveness of natural supplements on prevention and treatment of delayed onset muscle soreness and markers of muscle damage: a review of literature. Auc Kinanthropologgica, 57(1), 26-50. http://dx.doi.org/10.14712/23366052.2021.4
Davis, A.A., Tanner, E.A., Gary, M.A., & McFarlin, B.K. (2019). Curcumin and Boswellia Serrata Supplementation result in reduced Inflammation following Eccentric Leg Press Exercise. In International Journal of Exercise Science: Conference Proceedings, 2(11), 45. http://dx.doi.org/10.1249/01.mss.0000560764.09624.47
Delecroix, B., Leduc, C., Dawson, B., & Dupont, G. (2017). Curcumin and piperine supplementation and recovery following exercise induced muscle damage: A randomized controlled trial. Journal of Sports Science & Medicine, 16(1), 147. PMID: 28344463; PMCID: PMC5358025.
Dias, K.A., da Conceição, A.R., Oliveira, L.A., Pereira, S.M.S., Paes, S.D.S., Monte, L.F., ... & Della Lucia, C.M. (2021). Effects of curcumin supplementation on inflammatory markers, muscle damage, and sports performance during acute physical exercise in sedentary individuals. Oxidative Medicine and Cellular Longevity, 2021, 1-13. http://dx.doi.org/10.1155/2021/9264639
Fernández-Lázaro, D., Mielgo-Ayuso, J., Seco Calvo, J., Córdova Martínez, A., Caballero García, A., & Fernandez-Lazaro, C.I. (2020). Modulation of exercise-induced muscle damage, inflammation, and oxidative markers by curcumin supplementation in a physically active population: a systematic review. Nutrients, 12(2), 501. http://dx.doi.org/10.3390/nu12020501
Gholami, M., & Ardestani, M. (2018). Effects of quercetin supplementation on exercise induced inflammation and immune cell changes after exhausting swimming in adolescent girls. Asian Journal of Sports Medicine, 9(3), 1-7. [In Persian]. http://dx.doi.org/10.5812/asjsm.60157
He, Y., Yue, Y., Zheng, X., Zhang, K., Chen, S., & Du, Z. (2015). Curcumin, inflammation, and chronic diseases: how are they linked? Molecules, 20(5), 9183-9213. http://dx.doi.org/10.3390/molecules20059183
Insuan, O., Janchai, P., Thongchuai, B., Chaiwongsa, R., Khamchun, S., Saoin, S., ... & Vaithanomsat, P. (2021). Anti-inflammatory effect of pineapple rhizome bromelain through downregulation of the NF-κB-and MAPKs-signaling pathways in lipopolysaccharide (LPS)-stimulated RAW264. 7 cells. Current Issues in Molecular Biology, 43(1), 93-106. http://dx.doi.org/10.3390/cimb43010008
Jalali, M., Mahmoodi, M., Moosavian, S.P., Jalali, R., Ferns, G., Mosallanezhad, A., ... & Mosallanezhad, Z. (2020). The effects of ginger supplementation on markers of inflammatory and oxidative stress: a systematic review and meta‐analysis of clinical trials. Phytotherapy Research, 34(8), 1723-1733. [In Persian]. http://dx.doi.org/10.1002/ptr.6638
Kaiser, B.M., Stelzl, T., & Gedrich, K. (2020). Nutrition apps on focus: a qualitative assessment/ernährungs-apps im fokus: eine qualitätsbewertung. European Journal of Public Health Studies, 3(1), 9-33. http://dx.doi.org/10.46827/ejphs.v3i1.67
Kazemi, F., & Khani Sanij, E. (2019). Response of pro-inflammatory cytokines, IL-6 and IL-8 to short-term supplementation with ginger and acute exhaustive exercise in male athletes. Research in Medicine, 43(4), 222-227. [In Persian]. http://pejouhesh.sbmu.ac.ir/article-1-1953-en.html.
López Laval, I., Marques-Jiménez, D., Sitko, S., Mielgo-Ayuso, J., Calleja Gonzalez, J., & Velarde-Sotres, Á. (2022). Effects of ergo-nutritional strategies on recovery in combat sports disciplines. Nutrition Hospitalaria, 39(3), 652-662. http://dx.doi.org/10.20960/nh.03886
Lowery, R.P., Joy, J.M., Rathmacher, J.A., Baier, S.M., Fuller, J.C., Shelley, M.C., ... & Wilson, J.M. (2016). Interaction of beta-hydroxy-beta-methylbutyrate free acid and adenosine triphosphate on muscle mass, strength, and power in resistance trained individuals. Journal of Strength and Conditioning Research, 30(7), 1843-1854. http://dx.doi.org/10.1519/jsc.0000000000000482
Mahli, A., Koch, A., Fresse, K., Schiergens, T., Thasler, W.E., Schönberger, C., ... & Hellerbrand, C. (2018). Iso-alpha acids from hops (Humulus lupulus) inhibit hepatic steatosis, inflammation, and fibrosis. Laboratory Investigation, 98(12), 1614-1626. http://dx.doi.org/10.1038/s41374-018-0112-x
Markovic, M., Toskic, L., Kukic, F., Zaric, I., & Dopsaj, M. (2022). Sensitivity of field tests for assessment of wrestler’s specific fitness. Journal of Human Kinetics, 83(1), 267-76. http://dx.doi.org/10.2478/hukin-2022-0069
Melki, H., Bouzid, M.S., & Fadhloun, M. (2019). Correlation between Morphological and Functional Variables during a Specific Wrestling Test for Tunisian Cadet Greco-Roman Wrestlers. Journal of Physical Education and Sport, 19, 1282-1287. http://dx.doi.org/10.7752/jpes. 2019.s4186.
Mirzaei, B., Faryabi, I., & Yousefabadi, H.A. (2021). Time-Motion analysis of the 2017 Wrestling World Championships. Pedagogy of Physical Culture and Sports, 25(1), 24-30. [In Persian]. http://dx.doi.org/10.15561/26649837.2021.0104
Mirzaei, B., Rahmani Nia, F., & Mehrabani. J (2006). Determining the validity of Bruce and Kankani tests with gas analyzer method in estimating VO2max of national team wrestlers. Harakat Journal, 29, 111-122. [In Persian]. https://sid.ir/paper/30217/fa.
Moghadam Eftekhari, S., Vahidian Rezazadeh, M., Mogharnasi, M., & Karajibani, M. (2017). The effect of 8 weeks of aerobic exercises with ergometer and nettle extract supplementation on plasma levels of nesfatin-1 and C-reactive protein in overweight and obese women. Journal of Sport Biosciences, 9(1), 123-141. [In Persian]. https://doi.org/10.22059/jsb.2017.61939
Mótyán, J.A., Tóth, F., & Tozsér, J. (2013). Research applications of proteolytic enzymes in molecular biology. Biomolecules, 3(4), 923-942. http://dx.doi.org/10.3390/biom3040923
O’Fallon, K.S., Kaushik, D., Michniak-Kohn, B., Dunne, C.P., Zambraski, E.J., & Clarkson, P.M. (2012). Effects of quercetin supplementation on markers of muscle damage and inflammation after eccentric exercise. International Journal of Sport Nutrition and Exercise Metabolism, 22(6), 430-437. http://dx.doi.org/10.1123/ijsnem.22.6.430
Rawson, E.S., Miles, M.P., & Larson-Meyer, D.E. (2018). Dietary supplements for health, adaptation, and recovery in athletes. International Journal of Sport Nutrition and Exercise Metabolism, 28(2), 188-199. http://dx.doi.org/10.1123/ijsnem.2017-0340
Roschek Jr, B., Fink, R.C., McMichael, M., & Alberte, R.S. (2009). Nettle extract (Urtica dioica) affects key receptors and enzymes associated with allergic rhinitis. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 23(7), 920-926. http://dx.doi.org/10.1002/ptr.2763
Shakib, A., & Vakili, J. (2021). Effect of one-week supplementation of Citrulline-malate, L-arginine and their combination on CK, LDH and CRP levels in male wrestlers following simulated wrestling test. Medical Journal of Tabriz University of Medical Sciences, 43(2), 201-208. [In Persian]. http://dx.doi.org/10.34172/mj.2021.46
Shara, M., & Stohs, S.J. (2015). Efficacy and safety of white willow bark (Salix alba) extracts. Phytotherapy Research, 29(8), 1112-1116. http://dx.doi.org/10.1002/ptr.5377
Shing, C.M., Chong, S., Driller, M.W., & Fell, J.W. (2016). Acute protease supplementation effects on muscle damage and recovery across consecutive days of cycle racing. European Journal of Sport Science, 16(2), 206-212. http://dx.doi.org/10.1080/17461391.2014.1001878
Siddiqui, M.Z. (2011). Boswellia serrata, a potential antiinflammatory agent: an overview. Indian Journal of Pharmaceutical Sciences, 73(3), 255–261. http://dx.doi.org/10.4103/0250-474X.93507
Simioni, C., Zauli, G., Martelli, A.M., Vitale, M., Sacchetti, G., Gonelli, A., & Neri, L.M. (2018). Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging. Oncotarget, 9(24), 17181. http://dx.doi.org/10.18632/oncotarget.24729
Singh, P., Bhooshan Pandey, K., & Ibrahim Rizvi, S. (2016). Curcumin: the yellow molecule with pleiotropic biological effects. Letters in Drug Design & Discovery, 13(2), 170-177. http://dx.doi.org/10.2174/1570180812666150630184101
Tanabe, Y., Chino, K., Sagayama, H., Lee, H.J., Ozawa, H., Maeda, S., & Takahashi, H. (2019). Effective timing of curcumin ingestion to attenuate eccentric exercise-induced muscle soreness in men. Journal of Nutritional Science and Vitaminology, 65(1), 82-89. http://dx.doi.org/10.3177/jnsv.65.82
Tartibian, B., & Rezaei, B. (2021). Effect of HMB-FA supplementation on muscle damage indices in a simulated wrestling protocols in elite wrestlers. Sport Physiology, 13(50), 137-62. http://dx.doi.org/10.21203/rs.3.rs-2883051/v1
Tavakkolifar, B., Massoudi, M., & Zarringhalam, J. (2009). Activities of gum olibanum. Journal of Medicinal Plants, 8(32), 1-196. [In Persian]
Ulupınar, S., Özbay, S., Gençoglu, C., & Ince, I. (2021). performance differences between greco-roman and freestyle wrestlers: A systematic review and meta-analysis. Journal of Strength and Conditioning Research, 35(11), 3270-3279. http://dx.doi.org/10.1519/jsc.0000000000004129
Vahdat Poor, H., Shakerian, S., Alizadeh, A.A., & Fatemi Tabatabaei, S.R. (2016). The effect of short-term ginger supplementation on serum HSCRP and creatine kinase in response to exhaustive eccentric exercise in overweight girls. Jundishapur Scientific Medical Journal, 15(5), 451-550. [In Persian]. http://dx.doi.org/10.3402/fnr.v60.32613
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