Document Type : Original Article
Authors
Abstract
Background and Aim: The aim of this study was to determine the amount of total antioxidant capacity and hydrogen peroxide changes after an acute exercise in Wistar rats. Material and Methods: 14 male Wistar rats were randomly divided into two groups as control and acute exercise groups. Acute exercise intensity was set to 16 to 26 m/min for one hour, or until exhaustion on a treadmill running. Blood samples were collected 1 hour after exercise. Total antioxidant capacity and hydrogen peroxide were detected by FRAP and FOX-1 methods respectively. When the normal distribution of the data was revealed by Kolmogorov-Smirnov test, it is applied independent t-test at significance level of pResults: Results showed that TAC (p= 0.002) and H2O2 (p= 0.003) significantly increased in the acute exercise group as compared with control group. Conclusion: Although the level of hydrogen peroxide increased in response to the acute exercise, improved antioxidant capacity may be indicator of response of rats antioxidant system to the performed acute exercise.
Keywords
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10. Gross, M., Baum, O., Hoppeler, H., 2011. Antioxidant supplementation and endurance training: Win or loss? European Journal of Sport Science, vol. 11. no. 1, pp. 27-32.
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13. Kazeem, A., Olubayo, A., Ganiyu, A., 2012. Plasma Nitric Oxide and Acute Phase Proteins after Moderate and Prolonged xercises. Iranian Journal of Basic Medical Sciences, vol. 15. no. 1, pp. 602.
14. Kurkcu, R., 2010. The effects of short-term exercise on the parameters of oxidant and antioxidant system in handball
players. African Journal of Pharmacy and Pharmacology, vol. 4. no. 7, pp. 448-52.
15. Leelarungrayub, N., Sutabhaha, T., Pothongsunun, P., Chanarat, N., 2005. Exhaustive exercise test and oxidative stress response in athletic and sedentary subjects. Chiang Mai University Journal, vol. 4, pp. 183-90.
16. Maes, M., Fišar, Z., Medina, M., Scapagnini, G., et al. 2012. New drug targets in depression: inflammatory, cell-mediated immune, oxidative and nitrosative stress, mitochondrial, antioxidant, and neuroprogressive pathways. And new drug candidates—Nrf2 activators and GSK-3 inhibitors. Inflammopharmacology, vol. 20. no. 3, pp. 127-50.
17. Maes, M., Galecki, P., Chang, Y.S., Berk, M., 2011. A review on the oxidative and nitrosative stress (O&NS) pathways in major depression and their possible contribution to the (neuro) degenerative processes in that illness. Progress in Neuro-Psychopharmacology and Biological Psychiatry, vol. 35. no. 3, pp. 676-92.
18. Marius-Daniel, R., Stelian, S., Dragomir, C., 2010. The effect of acute physical exercise on the antioxidant status of the skeletal and cardiac muscle in the Wistar rat. Romanian Biotechnol Letters, vol. 15. no. 3, pp. 56-61.
19. Morillas-Ruiz, J., Villegas Garcia, J., Lopez, F., Vidal-Guevara, M., et al. 2006. Effects of polyphenolic antioxidants on exercise-induced oxidative stress. Clinical Nutrition, vol. 25. no. 3, pp. 444-53.
20. Noeman, S.A., Hamooda, H.E., Baalash, A.A., 2011. Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetology and Metabolic Syndrom, vol. 3. no. 1, pp. 17-17.
21. Paula, F.B., Gouvêa, C.M., Alfredo, P.P., Salgado, I., 2005. Protective action of a hexane crude extract of Pterodon
emarginatus fruits against oxidative and nitrosative stress induced by acute exercise in rats. BMC Complementary and
Alternative Medicine, vol. 5. no. 1, pp. 17.
22. Tauler, P., Sureda, A., Cases, N., Aguiló, A., et al. 2006. Increased lymphocyte antioxidant defences in response to
exhaustive exercise do not prevent oxidative damage. The Journal of Nutritional Biochemistry, vol. 17. no. 10, pp. 665-71.
23. Taysi, S., Oztasan, N., Efe, H., Polat, M., et al. 2008. Endurance training attenuates the oxidative stress due to acute
exhaustive exercise in rat liver. Acta Physiologica Hungarica, vol. 95. no. 4, pp. 337-47.
aerobic exercise session on the antioxidant defense system and lipid peroxidation of healthy young men. Journal of Sport in Biomotor Sciences, vol. 6. no. 2, pp. 39-50.
2. Aguiló, A., Tauler, P., Fuentespina, E., Tur, JA,. et al., 2005. Antioxidant response to oxidative stress induced by exhaustive exercise. Physiology & Behavior, vol. 84. no. 1, pp. 1-7.
3. Baghaiee, B., Tartibian, B., Baradaran, B., 1391. The relationship between total antioxidant status with creatine phosphokinase and hydrogen peroxide in the athlete girls; influenced by acute exercise training. Razi Journal of Medical Sciences, vol. 19. no. 95, pp. 43-35.
4. Benzie, I.F., Strain, J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, vol. 239. no. 1, pp. 70-76.
5. Bloomer, R.J., Fisher-Wellman, K.H., 2009. Systemic oxidative stress is increased to a greater degree in young, obese
women following consumption of a high fat meal. Oxidative Medicine and Cellular Longevity, vol. 2. no. 1, pp. 19-25.
6. De Castro, M., Cavalcanti Neto, F., Lima, L., Da Silva, F., et al., 2009. Production of free radicals and catalase activity during acute exercise training in young men. Biology of Sport, vol. 26. no. 2, pp. 113.
7. Ficicilar, H., Zergeroglu, A., Ersoz, G., Erdogan, A., et al., 2006. The effects of short-term training on platelet functions and total antioxidant capacity in rats. Physiological Research, vol. 55. no. 2, pp. 151.
8. Fisher-Wellman, K., Bloomer, R.J., 2009. Acute exercise and oxidative stress: a 30 year history. Dynamic Medicine, vol. 8. no. 1, pp. 1.
9. Ganie, S.A., Haq, E., Hamid, A., Masood, A., et al., 2011. Long dose exposure of hydrogen peroxide (H2O2) in albino rats and effect of Podophyllum hexandrum on oxidative stress. European Review for Medical and Pharmacological Sciences, vol. 15. no. 8, pp. 906-15.
10. Gross, M., Baum, O., Hoppeler, H., 2011. Antioxidant supplementation and endurance training: Win or loss? European Journal of Sport Science, vol. 11. no. 1, pp. 27-32.
11. Huang, C.C., Tsai, S.C., Lin, W.T., 2008. Potential ergogenic effects of L-arginine against oxidative and inflammatory stress induced by acute exercise in aging rats. Experimental Gerontology, vol. 43. no. 6, pp. 571-77.
12. Ji, L., Gomez-Cabrera, M., Steinhafel, N., Vina, J., 2004. Acute exercise activates nuclear factor (NF)-κB signaling pathway in rat skeletal muscle. The FASEB Journal, vol. 18. no. 13, pp. 1499-506.
13. Kazeem, A., Olubayo, A., Ganiyu, A., 2012. Plasma Nitric Oxide and Acute Phase Proteins after Moderate and Prolonged xercises. Iranian Journal of Basic Medical Sciences, vol. 15. no. 1, pp. 602.
14. Kurkcu, R., 2010. The effects of short-term exercise on the parameters of oxidant and antioxidant system in handball
players. African Journal of Pharmacy and Pharmacology, vol. 4. no. 7, pp. 448-52.
15. Leelarungrayub, N., Sutabhaha, T., Pothongsunun, P., Chanarat, N., 2005. Exhaustive exercise test and oxidative stress response in athletic and sedentary subjects. Chiang Mai University Journal, vol. 4, pp. 183-90.
16. Maes, M., Fišar, Z., Medina, M., Scapagnini, G., et al. 2012. New drug targets in depression: inflammatory, cell-mediated immune, oxidative and nitrosative stress, mitochondrial, antioxidant, and neuroprogressive pathways. And new drug candidates—Nrf2 activators and GSK-3 inhibitors. Inflammopharmacology, vol. 20. no. 3, pp. 127-50.
17. Maes, M., Galecki, P., Chang, Y.S., Berk, M., 2011. A review on the oxidative and nitrosative stress (O&NS) pathways in major depression and their possible contribution to the (neuro) degenerative processes in that illness. Progress in Neuro-Psychopharmacology and Biological Psychiatry, vol. 35. no. 3, pp. 676-92.
18. Marius-Daniel, R., Stelian, S., Dragomir, C., 2010. The effect of acute physical exercise on the antioxidant status of the skeletal and cardiac muscle in the Wistar rat. Romanian Biotechnol Letters, vol. 15. no. 3, pp. 56-61.
19. Morillas-Ruiz, J., Villegas Garcia, J., Lopez, F., Vidal-Guevara, M., et al. 2006. Effects of polyphenolic antioxidants on exercise-induced oxidative stress. Clinical Nutrition, vol. 25. no. 3, pp. 444-53.
20. Noeman, S.A., Hamooda, H.E., Baalash, A.A., 2011. Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetology and Metabolic Syndrom, vol. 3. no. 1, pp. 17-17.
21. Paula, F.B., Gouvêa, C.M., Alfredo, P.P., Salgado, I., 2005. Protective action of a hexane crude extract of Pterodon
emarginatus fruits against oxidative and nitrosative stress induced by acute exercise in rats. BMC Complementary and
Alternative Medicine, vol. 5. no. 1, pp. 17.
22. Tauler, P., Sureda, A., Cases, N., Aguiló, A., et al. 2006. Increased lymphocyte antioxidant defences in response to
exhaustive exercise do not prevent oxidative damage. The Journal of Nutritional Biochemistry, vol. 17. no. 10, pp. 665-71.
23. Taysi, S., Oztasan, N., Efe, H., Polat, M., et al. 2008. Endurance training attenuates the oxidative stress due to acute
exhaustive exercise in rat liver. Acta Physiologica Hungarica, vol. 95. no. 4, pp. 337-47.
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