Document Type : Original Article
Authors
1 Ph.D student in exercise physiology, Department of Sport Science, Islamic Azad University, Bojnourd, Iran.
2 Assistant Professor of exercise physiology, Department of Sport Science, Islamic Azad University, Bojnourd, Iran.
3 Professor of Medical Physiology, Department of Physiology, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract
Background and Aim: Inflammatory bowel disease (IBD) is a group of chronic gastrointestinal diseases that pose a challenge to its treatment. This study aimed to investigate the effect of aerobic swimming exercise and in combination with hydrogen-rich water (HRW) on clinical symptoms, histological changes and oxidative stress markers in an animal model of colitis. Materials and Methods: Thirty male C57BL6 mice were divided into control, swimming exercise, colitis, colitis+swimming exercise, colitis+swimming exercise+ colitis groups. At the end of study, colon weight and length, spleen weight, disease activity index, histological changes and oxidative stress markers in colon tissue were evaluated. Data were analyzed using one-way or two-way ANOVA and p<0.05 was considered statistically significant. Results: Results showed that there were no significant differences between control and swimming exercised groups. But, in colitis animals, the disease activity index (such as weight loss, bleeding, and rectal protrusion) in exercised group decreased significantly compared to the colitis group; which showed a further decrease in the combined group (p<0.001). Moreover, colon length was also increased in exercised groups compared to the colitis group (p<0.05). Histological indices (such as leukocyte infiltration) and fibrosis were significantly decreased in exercsied groups compared to the colitis group (p<0.01). Colitis animals had higher malondialdehyde and lower antioxidative markers (superoxihde dismutase, catalase, total thiol) compared to the control group; while exercise and exercise plus HRW significantly improved antioxidative factors. Conclusion: The results showed that swimming exercise especially in combination with HRW could improve clinical symptoms and histological changes of colon tissue in colitis animals and it can be considered as treatment strategy in patients with colitis.
Keywords
Asgharzadeh, F., Tarnava, A., Mostafapour, A., Khazaei, M., & LeBaron, T. W. (2022). Hydrogen-rich water exerts anti-tumor effects comparable to 5-fluorouracil in a colorectal cancer xenograft model. World Journal of Gastrointestinal Oncology, 14(1), 242-252. https://doi.org/10.4251/wjgo.v14.i1.242
Binabaj, M.M., Asgharzadeh, F., Avan, A., Rahmani, F., Soleimani, A., Parizadeh, M. R., ... & Hassanian, S.M. (2019). EW‐7197 prevents ulcerative colitis‐associated fibrosis and inflammation. Journal of Cellular Physiology, 234(7), 11654-11661. https://doi.org/10.1002/jcp.27823
Choi, J.H., Chung, K.S., Jin, B.R., Cheon, S.Y., Nugroho, A., Roh, S.S., & An, H.J. (2017). Anti-inflammatory effects of ethanol extract of Aster glehni via inhibition of NF-κB activation in mice with DSS-induced colitis. Food & Function, 8(7), 2611-2620. https://doi.org/10.1039/c7fo00369b
Edelmann, F., Gelbrich, G., Dungen, H.D., Frohling, S., Wachter, R., Stahrenberg, R., ... & Pieske, B. (2011). Exercise training improves exercise capacity and diastolic function in patients with heart failure with preserved ejection fraction: results of the Ex-DHF (exercise training in dastolic hart filure) pilot study. Journal of American College Cardiology, 58(17), 1780-1791. https://doi.org/10.1016/j.jacc.2011.06.054
Iida, A., Nosaka, N., Yumoto, T., Knaup, E., Naito, H., Nishiyama, C., . . . & Nakao, A. (2016). The Clinical application of hydrogen as a medical treatment. Acta Medica Okayama, 70(5), 331-337. https://doi.org/10.18926/AMO/54590
Kajisa, T., Yamaguchi, T., Hu, A., Suetake, N., & Kobayashi, H. (2017). Hydrogen water ameliorates the severity of atopic dermatitis-like lesions and decreases interleukin-1β, interleukin-33, and mast cell infiltration in NC/Nga mice. Saudi Medical Journal, 38(9), 928. https://doi.org/10.15537/smj.2017.9.20807
Kajiya, M., Silva, M. J., Sato, K., Ouhara, K., & Kawai, T. (2009). Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochemical and Biophysical Research Communications, 386(1), 11-15. https://doi.org/10.1016/j.bbrc.2009.05.117
Kajisa, T., Yamaguchi, T., Hu, A., Suetake, N., & Kobayashi, H. (2017). Hydrogen water ameliorates the severity of atopic dermatitis-like lesions and decreases interleukin-1β, interleukin-33, and mast cell infiltration in NC/Nga mice. Saudi Medical Journal, 38(9), 928. https://doi.org/10.15537/smj.2017.9.20807
Kaser, A., Zeissig, S., & Blumberg, R.S. (2010). Inflammatory bowel disease. Annual Review of Immunology, 28, 573-621. https://doi.org/10.1146/annurev-immunol-030409-101225
Kim, J.J., Shajib, M.S., Manocha, M.M., & Khan, W.I. (2012). Investigating intestinal inflammation in DSS-induced model of IBD. Journal of Visualized Experiments,1(60), 3678. https://doi.org/10.3791/3678
Kobayashi, T., Siegmund, B., Le Berre, C., Wei, S. C., Ferrante, M., Shen, B., ... & Hibi, T. (2020). Ulcerative colitis. Nature Reviews Disease Primers, 6(1), 74. https://doi.org/10.1038/s41572-020-0205-x
LeBaron, T.W., Asgharzadeh, F., Khazaei, M., Kura, B., Tarnava, A., & Slezak, J. (2021). Molecular hydrogen is comparable to sulfasalzine as a treatment for DSS- induced colitis in mice. EXCLI Journal, 20, 1106-1117. https://doi.org/10.17179/excli2021-3762
Li, Y., Wang, Z., Lian, N., Wang, Y., Zheng, W., Xie, K. (2021). Molecular Hydrogen: A Promising Adjunctive Strategy for the Treatment of the COVID-19. Frontiers in Medicine, 8, 671215. https://doi.org/10.3389/fmed.2021.671215
Liang, I.C., Ko, W.C., Hsu, Y.J., Lin, Y.R., Chang, Y.H., Zong, X.H., ... & Hung, C.F. (2021). The anti-inflammatory effect of hydrogen gas inhalation and its influence on laser-induced choroidal neovascularization in a mouse model of neovascular age-related macular degeneration. International Journal of Molecular Sciences, 22(21),12049. https://doi.org/10.3390/ijms222112049
Madesh, M., & Balasubramanian, K. (1998). Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry & Biophysics, 35(3), 184-188. PMID: 9803669
Manson, J.E., Greenland, P., LaCroix, A.Z., Stefanick, M.L., Mouton, C.P., Oberman, A., . . . & Siscovick, D.S. (2002). Walking compared with vigorous exercise for the prevention of cardiovascular events in women. New England Journal of Medicine, 347(10), 716-725. https://doi.org/10.1056/nejmoa021067
Nakao, A., Toyoda, Y., Sharma, P., Evans, M., & Guthrie, N. (2010). Effectiveness of hydrogen rich water on antioxidant status of subjects with potential metabolic syndrome—an open label pilot study. Journal of Clinical Biochemistry and Nutrition, 46(2), 140-149. https://doi.org/10.3164/jcbn.09-100
Nogueira, J.E., Passaglia, P., Mota, C.M.D., Santos, B.M., Batalhao, M.E., Carnio, E.C., & Branco, L.G. S. (2018). Molecular hydrogen reduces acute exercise-induced inflammatory and oxidative stress status. Free Radical Biology and Medicine, 129, 186-193. https://doi.org/10.1016/j.freeradbiomed.2018.09.028
Obermeier, F., Kojouharoff, G., Hans, W., Schölmerich, J., Gross, V., & Falk, W. (1999). Interferon-gamma (IFN-γ)-and tumour necrosis factor (TNF)-induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium (DSS)-induced colitis in mice. Clinical & Experimental Immunology, 11, 238, .2-6. https://doi.org/10.1046/j.1365-2249.1999.00878.x
Park, Y.H., Kim, N., Shim, Y.K., Choi, Y.J., Nam, R.H., Choi, Y.J., ... & Lee, C.M. (2015). Adequate dextran sodium sulfate-induced colitis model in mice and effective outcome measurement method. Journal of Cancer Prevention, 20(4), 260. https://doi.org/10.15430/jcp.2015.20.4.260
Qin, L., Yao, Z.Q., Chang, Q., Zhao, Y.L., Liu, N.N., Zhu, X.S., . . . & Li, J.T. (2017). Swimming attenuates inflammation, oxidative stress, and apoptosis in a rat model of dextran sulfate sodium-induced chronic colitis. Oncotarget, 8(5), 7391-7404. https://doi.org/10.18632/oncotarget.14080
Sallam, N., & Laher, I. (2016). Exercise modulates oxidative stress and inflammation in aging and cardiovascular diseases. Oxidative Medicine and Cellular Longevity, 2016, 7239639. https://doi.org/10.1155/2016/7239639
Sun, Y., Cui, D., Zhang, Z., Zhang, T., Shi, J., Jin, H., ... & Ding, S. (2016). Attenuated oxidative stress following acute exhaustive swimming exercise was accompanied with modified gene expression profiles of apoptosis in the skeletal muscle of mice. Oxidative Medicine and Cellular Longevity, 2016, 8381242. https://doi.org/10.1155/2016/8381242
Szalai, Z., Szasz, A., Nagy, I., Puskas, L.G., Kupai, K., Kiraly, A., ... & Varga, C. (2014). Anti-inflammatory effect of recreational exercise in TNBS-induced colitis in rats: role of NOS/HO/MPO system. Oxidative Medicine and Cellular Longevity, 2014, 925981. https://doi.org/10.1155/2014/925981
Xiao, H.w., Li, Y., Luo, D., Dong, J.l., Zhou, L.x., Zhao, S.y., ... & Fan, S.J. (2018). Hydrogen-water ameliorates radiation-induced gastrointestinal toxicity via MyD88’s effects on the gut microbiota. Experimental & Molecular Medicine, 50(1), e433. https://doi.org/10.1038/emm.2017.246
Zheng, G., Qiu, P., Xia, R., Lin, H., Ye, B., Tao, J., & Chen, L. (2019). Effect of aerobic exercise on inflammatory markers in healthy middle-aged and older adults: A systematic review and meta-analysis of randomized controlled trials. Frontiers in Aging Neuroscience, 26(11), 98. https://doi.org/10.3389/fnagi.2019.00098
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