The effect of eight weeks of incremental aerobic training on gene expression of asprosin in the heart and serum malondialdehyde of male rats diabetic

Mohajer, Mona; Haghshenas, Ruhollah

doi:10.22077/jpsbs.2023.5403.1739

The effect of eight weeks of incremental aerobic training on gene expression of asprosin in the heart and serum malondialdehyde of male rats diabetic

Articles in Press

Document Type : Original Article

Authors

Mona Mohajer ¹

Ruhollah Haghshenas ²

¹ Master of Exercise Physiology, Department of Sports Science, Semnan University, Semnan, Iran

² Associate Professor of Exercise Physiology, Department of Sports Science, Faculty of Humanities, Semnan University, Semnan, Iran

10.22077/jpsbs.2023.5403.1739

Abstract

Background and Aim: Today, a new protein called asprosin has been discovered, which plays a role in regulating blood sugar. The aim of this study was to evaluate the effect of eight weeks of aerobic training on gene expression of asprosin and malondialdehyde (MDA) in heart tissue of male diabetes rats. Materials and Methods: Thirty-two adults male Wistar rats were randomly divided into four groups (every group eight rat): control; Exercise; Diabetes; And aerobic exercise and diabetes. After making the rats diabetic by injecting Strepotozocin, the aerobic training protocol was performed for 8 weeks and 5 days a week, and 48 hours after the last training session, cardiac tissue and blood samples were taken from the rats. ELISA method was used to measure protein expression and MDA, and RT-PCR method was used to measure gene expression. Kruskal-Wallis statistical tests, ANOVA and Tukey's post hoc test were used to analyze the data at a significance level of p<0.05. Results: Heart tissue asprosin gene expression (p<0.001) and serum MDA levels (p<0.001) increased significantly after diabetes, but aerobic training had no significant effect on them (p>0.05).Conclusion: Since the expression of asprosin gene has recently been detected in the heart tissue, it seems that more studies are needed to know its function in the heart tissue.

Keywords

Aerobic training

Diabetes

Asprosin

Obesity

Chronic inflammation