Document Type : Original Article
Authors
1 Professor at Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Allameh Tabataba’i University, Tehran, Iran.
2 MS.c in Exercise Physiology, Faculty of Physical Education and Sports Sciences, Allameh Tabataba’i University, Tehran, Iran.
3 Assistant Professor at Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Allameh Tabataba’i University, Tehran, Iran.
Abstract
Background and Aim: It seems that the use of nutritional supplements can affect the secretion of anabolic and catabolic hormones during exercise-recovery process. The purpose of the present study was to determine the effect of beta-hydroxy beta methyl butyrate supplement in free acid form (HMB-FA) on hormonal responses and recovery in the wrestling-specific protocol (WSP) in wrestlers for one night and one day. Materials and Methods: Twenty male wrestlers were selected and randomly divided into two groups: supplement-exercise (n=10) and placebo-exercise (n=10) groups. Each of the groups received a supplement or placebo at 3 g/day. The subjects repeated the WSP five times, each consisting of four sections. To measure serum insulin-like growth factor-1 (IGF-1), and cortisol (Cort), IGF-1/Cort ratio blood samples were taken before and after taking the supplement, immediately after the first repetition of the WSP, immediately after the third repetition of the WSP and immediately after the fifth repetition of the WSP. The perceived recovery status (PRS) was assessed immediately before the first, second, third, fourth, and fifth repetitions of the WSP. Results were extracted using analysis of covariance and analysis of variance with repeated measures at a significance level of p<0.05. Results: The results showed that serum levels of IGF-1 after supplementation (p=0.001) and after the first (p=0.004), third (p=0.004) and fifth (p=0.01) of the WSP were significantly higher in HMB-FA group than placebo group. Moreover, serum levels of IGF-1/Cort after supplementation (p=0.001) and after the first (p=0.001), third (p=0.003) and fifth (p=0.001) of the WSP were significantly increased in the HMB-FA group as compare to the placebo group. However, cortisol indicated a higher increase in the placebo group than HMB-FA group after supplementation (p=0.006) and also after first (p=0.002), third (p=0.008) and fifth (p=0.002) of WSP. On the other hand, PRS in the placebo group showed a greater decrease than the HMB-FA group before the first, second, third, fourth, and fifth of WSP (p<0.05). Conclusion: It seems that HMB-FA supplementation improves hormonal responses during the simulated wrestling protocol.
Keywords
Akbarnejad, A., Ravasi, A., Aminian Razavi, T., & Noor Mohammadi, I. (2006). The effect of taking glutamine and creatine supplements on the immune system and athletic performance of elite wrestlers after a period of acute weight loss. Harakat, 27, 173- 188. [In Persian]
Amirsasan, R., & Hessaei, J. (2018). Effect of short term supplementation of two different doss of colostrum on cell damage indicators in male wrestlers. Iranian Journal of Nutrition Sciences & Food Technology, 13(1), 21-30.
Anderson, L.J., Tamayose, J.M., & Garcia, J.M. (2018). Use of growth hormone, IGF-I, and insulin for anabolic purpose: pharmacological basis, methods of detection, and adverse effects. Molecular and Cellular Endocrinology, 464, 65-74.
Anthony, T.G. (2016). Mechanisms of protein balance in skeletal muscle. Domestic Animal Endocrinology, 56, S23-S32.
Asadi, A., Arazi, H., & Suzuki, K. (2017). Effects of β-hydroxy-β-methylbutyrate-free acid supplementation on strength, power and hormonal adaptations following resistance training. Nutrients, 9(12), 1316.
Assad, M., Zoghi, R., & Fashi, M. (2015). The effect of eight weeks of resistance training with HMB supplementation on hormonal changes and testosterone in non-athlete men. Journal of Alborz University of Medical Sciences, 5(3), 193-187. [In Persian]
Bagherpoor goll, T., Amirsasan, R., & Hessaei, J. (2018). Effect of Short term supplementation of two different doses of Colostrum on cell damage indicators in male wrestlers. Iranian Journal of Nutrition Sciences & Food Technology,13(1), 21-30. [In Persian]
Branco, B.H.M., Fukuda, D.H., Andreato, L.V., da Silva Santos, J.F., Esteves, J. V.D.C., & Franchini, E. (2016). The effects of hyperbaric oxygen therapy on post-training recovery in Jiu-Jitsu athletes. PloS One, 11(3), e0150517.
Correia, A.L.M., de Lima, F.D., Bottaro, M., Vieira, A., da Fonseca, A.C., & Lima, R.M. (2018). Pre-exercise β-hydroxy-β-methylbutyrate free-acid supplementation improves work capacity recovery: a randomized, double-blinded, placebo -controlled study. Applied Physiology, Nutrition, and Metabolism, 999, 1-6.
Dashti, M.H. (2011). The effect of programmed exercise on body compositions and heart rate of 11-13 years-old male students. Zahedan Medical Sciences Research Journal (Tabib Sharq), 13, 40-43. [In Persian]
Demirhan, B., Yaman, M., Cengiz, A., Saritas, N., & Günay, M. (2015). Comparison of ice massage versus cold-water immersion on muscle damage and DOMS levels of elite wrestlers. The Anthropologist, 19(1), 123-129.
Eichmann, B., Kobes, J., Sherve, C., Aho, A., & Saghiv, M. (2017). The university of Mary wrestling anaerobic performance test: A new wrestling-specific Protocol. International Journal of Wrestling Science, 7(1-2), 15-20.
Eryılmaz, S.K., Aslankeser, Z., Özdemir, Ç., Özgünen, K., & Kurdak, S. (2019). The effect of 30-m repeated sprint exercise on muscle damage indicators, serum insulin-like growth factor-Iand cortisol. Biomedical Human Kinetics, 11(1), 151-157.
Fortes, L.S., Lira, H.A., Mendonça, L.C., Paes, P.P., Vianna, J.M., & Pérez, A.J. (2019). Effect of body weight reduction on stress and recovery among Brazilian Judokas. International Journal of Sport and Exercise Psychology, 17(1), 74-84.
Hamedchaman, N., Tofighi, A., & Toloueiazar, J. (2020). Comparison of the effects of 8 weeks of complex training and HMB supplements on IL-15 levels, cortisol, and some physiological and functional variables of male basketball players. Journal of Sport Biosciences, 12(1), 31-51. [In Persian]
Hammarqvist, F., Wennström, I., & Wernerman, J. (2009). Effects of growth hormone and insulin-like growth factor-1 on postoperative muscle and substrate metabolism. Journal of Nutrition and Metabolism, (2010), 1-9.
Holeček, M. (2017). Beta‐hydroxy‐beta‐methylbutyrate supplementation and skeletal muscle in healthy and muscle‐wasting conditions. Journal of Cachexia, Sarcopenia and Muscle, 8(4), 529-541.
Kaiser, B. M., Stelzl, T., & Gedrich, K. (2020). Nutrition apps on foccus: A quqlitative assessment. European Journal of Public Health Studies, 3(1), 9-32.
Kellmann, M. (2010). Preventing overtraining in athletes in high‐intensity sports and stress/recovery monitoring. Scandinavian Journal of Medicine & Science in Sports, 20, 95-102.
Knowles, O.E., Aisbett, B., Main, L.C., Drinkwater, E.J., Orellana, L., & Lamon, S. (2019). Resistance training and skeletal muscle protein metabolism in Eumenorrheic Ffmales: Implications for researchers and practitioners. Sports Medicine, 49, 1637–1650.
Laurent, C.M., Green, J.M., Bishop, P.A., Sjökvist, J., Schumacker, R.E., Richardson, M.T., & Curtner-Smith, M. (2011). A practical approach to monitoring recovery: development of a perceived recovery status scale. The Journal of Strength & Conditioning Research, 25(3), 620-628.
Lowery, R.P., Joy, J.M., Rathmacher, J.A., Baier, S.M., Fuller, J.C., Shelley, M. C., ... & Wilson, J.M. (2016). Interaction of beta-hydroxy-beta-methylbutyrate free acid and adenosine triphosphate on muscle mass, strength, and power in resistance trained individuals. Journal of Strength and Conditioning Research, 30(7), 1843-1854.
Maleki, B.H., Tartibian, B., Mooren, F.C., Nezhad, F.Y., & Yaseri, M. (2016). Saffron supplementation ameliorates oxidative damage to sperm DNA following a 16-week low-to-intensive cycling training in male road cyclists. Journal of Functional Foods, 21, 153-166.
Martins, P., & Pedro, S. (2017). Motivational regulations and recovery in olympic wrestlers. International Journal of Wrestling Science, 7(1-2), 27-34.
Melanorozi. A., & Hesar Kooshki, M. (2019). Evaluation of two methods of wrestling training on serum cortisol and testosterone levels in wrestlers. Fifth National Conference on Sports Science and Physical Education, Tehran Iran, March 11, 2019. Sportconf 05_096. [In Persian]
Mirzaei, B., Ghahremani Moghaddam, M., & Alizaee Yousef Abadi, H. (2017). Analysis of energy systems in Greco-Roman and freestyle wrestlers who participated in the 2015 and 2016 world championships. International Journal of Wrestling Science, 7(1-2), 35-40. [In Persian]
Mirzaei, B., Rahmani Nia, F., & Mehrabani. J (2006). Determining the validity of Bruce and Conconi tests with gas analyzer method in estimating VO2max of national team wrestlers. Harkat, 29, 111-122. [In Persian]
Moore, D. . (2019). Maximizing post-exercise anabolism: the case for relative protein intakes. Frontiers in Nutrition, 6, (147) 1-40.
Nikooie, R., Cheraghi, M., & Mohamadipour, F. (2017). Physiological determinants of wrestling success in elite Iranian senior and junior Greco-Roman wrestlers. The Journal of Sports Medicine and Physical Fitness, 57(3), 219-226.
Piskin, E., Bayraktaroglu, T., Yamaner, F., Gumus, M., & Tamer, K. (2013). Growth and pubertal development in adolescent male wrestlers. In 15th European Congress of Endocrinology, 58(6), 852-6.
Qasem Nian, A.A,. Noor Mohammadi, E,. Azad, A. (2017). Evaluation of changes in insulin-like growth factor-1 (IGF-I), serum cortisol and performance of female wheelchair basketball players after 8 weeks of upper body strength training. Journal of Medical Science Studies Journal of Urmia University of Medical Sciences, 27(10), 836-847. [In Persian]
Salimi Avansar, M. (2017). Effect of HIIT training on the levels of Omentin-1 and body composition characteristics in sedentary obese men. Journal of Sport and Exercise Physiology, 10(2), 59-68. [In Persian]
Sahin, I. (2013). Recovery dynamics following specific wrestling exercise. International Journal of Academic Research, 5(4), 108-112.
Saki, B., & Gaieni, A. (2012). The effect of short-term use of HMB on AST, ALT, AP and serum urea levels after one session of intense resistance exercise in non-athlete male students. Journal of Isfahan Medical School, 30 (190), 695-704.
Sato, T., Ito, Y., & Nagasawa, T. (2013). Regulation of skeletal muscle protein degradation and synthesis by oral administration of lysine in rats. Journal of Nutritional Science and Vitaminology, 59(5), 412-419.
Sharma, L., Hussain, M.E., & Verma, S. (2017). Effect of recovery modalities on blood lactate clearance. Saudi Journal of Sports Medicine, 17(2), 65.
Sheikholeslami vatani, D., & Ahmadi, K. (2015). The effect of short-term use of HMB and creatine supplements on oxidative and antioxidant indices in people trained after acute resistance activity. Journal of Exercise Physiology and Physical Activity, 10(1), 78 - 71.
Shirvani, H., Rahmati-Ahmadabad, S., Kowsari, E., Fry, H., Kazemi, M., & Kaviani, M. (2020). Effects of 2-week HMB-FA supplementation with or without eccentric resistance exercise on expression of some genes related to muscle protein turnover and serum irisin and IGF-1 concentrations. Gene, 760(15), 145018.
Sikorski, E.M., Wilson, J.M., Lowery, R.P., Joy, J.M., Laurent, C.M., Wilson, S.M., ... & Gilchrist, P. (2013). Changes in perceived recovery status scale following high-volume muscle damaging resistance exercise. The Journal of Strength & Conditioning Research, 27(8), 2079-2085.
Silva, V.R., Belozo, F.L., Micheletti, T.O., Conrado, M., Stout, J.R., Pimentel, G.D., & Gonzalez, A.M. (2017). β-Hydroxy-β-methylbutyrate free acid supplementation may improve recovery and muscle adaptations after resistance training: A systematic review. Nutrition Research, 45, 1-9.
Tianlong, D., & Sim, Y.J. (2019). Effects of different recovery methods on postboxing sparring fatigue substances and stress hormones. Journal of Exercise Rehabilitation, 15(2), 258.
Tinsley, G.M., Givan, A.H., Graybeal, A.J., Villarreal, M.I., & Cross, A.G. (2018). β-Hydroxy β-methylbutyrate free acid alters cortisol responses, but not myofibrillar proteolysis, during a 24-h fast. British Journal of Nutrition, 119(5), 517-526.
Teixeira, F.J., Matias, C.N., Monteiro, C.P., Valamatos, M.J., Reis, J.F., Tavares, F., ... & Phillips, S.M. (2019). Leucine metabolites do not enhance training-induced performance or muscle thickness. Medicine and Science in Sports and Exercise, 51(1), 56-64.
Townsend, J.R., Hoffman, J.R., Gonzalez, A.M., Jajtner, A.R., Boone, C.H., Robinson, E.H., & Stout, J.R. (2015). Effects of β-hydroxy-β-methylbutyrate free acid ingestion and resistance exercise on the acute endocrine response. International Journal of Endocrinology, 2015, 1-7.
Wilson, J.M., Fitschen, P.J., Campbell, B., Wilson, G.J., Zanchi, N., Taylor, L., … Antonio, J. (2013a). International society of sports nutrition position stand: beta-hydroxy-beta-methylbutyrate (HMB). Journal of the International Society of Sports Nutrition, 10(1), 6.
Wilson, J.M., Lowery, R.P., Joy, J.M., Walters, J.A., Baier, S.M., Fuller, J.C., ... & Duncan, N.M. (2013b). β-Hydroxy-β-methylbutyrate free acid reduces markers of exercise-induced muscle damage and improves recovery in resistance-trained men. British Journal of Nutrition, 110(3), 538-544.
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