Document Type : Original Article
Authors
1 PhD Student in Exercise Physiology, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran..
2 Assistant Professor, Department of Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran.
3 Associate Professor, Department of Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran.
4 Assistant Professor, Department of Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran.
Abstract
Background and Aim: The carbohydrate mouth rinse (CMR) has been proposed as a strategy to boost athletic performance. The present study aimed to investigate the effect of CMR on the electrical activity of the quadriceps muscle during a sub-maximal cycling task within different phases of the menstrual cycle in female athletes. Materials and Methods: Ten active females came to the laboratory on five different times. In the first session, peak power output was measured and during the second to fifth visit (5th and 12th days of the follicular phase and 17th and 22th days of the luteal phase) each subject completed a one-hour cycling task at 65% of peak power output under either CMR or placebo condition in each phase. Before and during every 15 minutes of the trial, participants rinsed their mouths with carbohydrate or placebo solution. Lactate, and blood glucose were measured before and immediately after the trial. Moreover, during the task, heart rate and rating of perceived exertion also were recorded every 10 minutes, and the electrical activity of the Rectus Femoris, Vastus Lateralis, and Vastus Medialis muscles was recorded every 15 minutes. A repeated measures ANOVA and Bonferonni tests were used for statistical analysis at the p<0.05 significant level. Results: The electrical activity of the Vastus Lateralis muscle at times 45 (p=0.04) and 60 (p=0.04) and, the Rectus Femoris muscle at time 45 (p=0.01) in the follicular phase under CMR condition was significantly higher than the same times in the luteal phase. Conclusion: These results indicated the possible interference effect of menstrual phases on CMR-induced changes in muscular performance. It seems that maintaining the electrical activity of the quadriceps muscle under CMR condition in the follicular phase as compared to the luteal phase could be related to sex hormone levels.
Keywords
Bastos-Silva, V.J., Melo, A.D.A., Lima-Silva, A.E., Moura, F.A., Bertuzzi, R., & De Araujo, G.G. (2016). Carbohydrate mouth rinse maintains muscle electromyographic activity and increases time to exhaustion during moderate but not high-intensity cycling exercise. Nutrients, 8(3), 49.
Becker, J.B., & Cha, J.-H. (1989). Estrous cycle-dependent variation in amphetamine-induced behaviors and striatal dopamine release assessed with microdialysis. Behavioural Brain Research, 35(2), 117-125.
Becker, J.B., Robinson, T.E., & Lorenz, K.A. (1982). Sex difference and estrous cycle variations in amphetamine-elicited rotational behavior. European Journal of Pharmacology, 80(1), 65-72.
Bieuzen, F., Lepers, R., Vercruyssen, F., Hausswirth, C., & Brisswalter, J. (2007). Muscle activation during cycling at different cadences: effect of maximal strength capacity. Journal of Electromyography and Kinesiology, 17(6), 731-738.
Borg, G.A. (1982). Psychophysical bases of perceived exertion. Medicine & Science in Sports & Exercise, 14, 377-381.
Campbell, S., Angus, D., & Febbraio, M. (2001). Glucose kinetics and exercise performance during phases of the menstrual cycle: effect of glucose ingestion. American Journal of Physiology-Endocrinology And Metabolism, 281(4), E817-E825.
Carter, J.M., Jeukendrup, A.E., & Jones, D.A. (2004). The effect of carbohydrate mouth rinse on 1-h cycle time trial performance. Medicine and Science in Sports and Exercise, 36(12), 2107-2111.
Carter, J.M., Jeukendrup, A. E., Mann, C.H., & Jones, D.A. (2004). The effect of glucose infusion on glucose kinetics during a 1-h time trial. Medicine and Science in Sports and Exercise, 36(9), 1543-1550.
Chambers, E., Bridge, M., & Jones, D. (2009). Carbohydrate sensing in the human mouth: effects on exercise performance and brain activity. The Journal of Physiology, 587(8), 1779-1794.
Chryssanthopoulos, C., Ziaras, C., Oosthuyse, T., Lambropoulos, I., Giorgios P, P., Zacharogiannis, E., ... & Maridaki, M. (2018). Carbohydrate mouth rinse does not affect performance during a 60-min running race in women. Journal of Sports Sciences, 36(7), 824-833.
Clarke, N.D., Thomas, J.R., Kagka, M., Ramsbottom, R., & Delextrat, A. (2017). No dose-response effect of carbohydrate mouth rinse concentration on 5-km running performance in recreational athletes. Journal of Strength and Conditioning Research, 31(3), 715-720.
Creutz, L.M., & Kritzer, M.F. (2004). Mesostriatal and mesolimbic projections of midbrain neurons immunoreactive for estrogen receptor beta or androgen receptors in rats. Journal of Comparative Neurology, 476(4), 348-362.
Devries, M.C., Hamadeh, M.J., Phillips, S.M., & Tarnopolsky, M.A. (2006). Menstrual cycle phase and sex influence muscle glycogen utilization and glucose turnover during moderate-intensity endurance exercise. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 291(4), R1120-R1128.
Dreher, J.C., Schmidt, P.J., Kohn, P., Furman, D., Rubinow, D., & Berman, K.F. (2007). Menstrual cycle phase modulates reward-related neural function in women. Proceedings of the National Academy of Sciences, 104(7), 2465-2470.
Fares, E.-J., & Kayser, B. (2011). Carbohydrate mouth rinse effects on exercise capacity in pre-and postprandial States. Journal of Nutrition and Metabolism, 2011.
Ferreira, A.M., Farias-Junior, L.F., Mota, T.A., Elsangedy, H.M., Marcadenti, A., Lemos, T.M., . . . & Fayh, A.P. (2018). The effect of carbohydrate mouth rinse on performance, biochemical and psychophysiological variables during a cycling time trial: a crossover randomized trial. Journal of the International Society of Sports Nutrition, 15(1), 23.
Fraga, C., Velasques, B., Koch, A.J., Machado, M., Paulucio, D., Ribeiro, P., & Pompeu, F.A.M.S. (2017). Carbohydrate mouth rinse enhances time to exhaustion during treadmill exercise. Clinical Physiology and Functional Imaging, 37(1), 17-22.
Gant, N., Stinear, C.M., & Byblow, W D. (2010). Carbohydrate in the mouth immediately facilitates motor output. Brain Research, 1350, 151-158.
Hackney, A. (1999). Influence of oestrogen on muscle glycogen utilization during exercise. Acta physiologica scandinavica, 167(3), 273.
Hematfar, A. & Ghoorehdan, L. (2016). A comparison effects of carbohydrate alone and carbohydrate with L-Arginine supplementation on serum insulin and glucose levels during recovery times after aerobic activity. Journal of Practical Studies of Biosciences in Sport, 3(6), 21-30. [In Persian]
Hessemer, V., & Bruck, K. (1985). Influence of menstrual cycle on thermoregulatory, metabolic and heart rate responses to exercise at night. Journal of Applied Physiology, 59(6), 1911–1917.
Hermens, H.J., Freriks, B., Disselhorst-Klug, C., & Rau, G. (2000). Development of recommendations for SEMG sensors and sensor placement procedures. Journal of Electromyography and Kinesiology, 10(5), 361-374.
Inghilleri, M., Conte, A., Curra, A., Frasca, V., Lorenzano, C., & Berardelli, A. (2004). Ovarian hormones and cortical excitability. An rTMS study in humans. Clinical Neurophysiology, 115(5), 1063-1068.
Jamshidi Hossein Abadi, M., Jamshidi Hossein Abadi, A., & Behpoor, N. (2015). The effect of L-arginine supplementation on serum growth hormone, lactate and glucose responses to aerobic activity in boy students. Journal of Practical Studies of Biosciences in Sport, 3(5), 9-22. [In Persian]
Jeffers, R., Shave, R., Ross, E., Stevenson, E.J., & Goodall, S. (2015). The effect of a carbohydrate mouth-rinse on neuromuscular fatigue following cycling exercise. Applied Physiology, Nutrition, and Metabolism, 40(6), 557-564.
Jentjens, R.L., Underwood, K., Achten, J., Currell, K., Mann, C.H., & Jeukendrup, A.E. (2006). Exogenous carbohydrate oxidation rates are elevated after combined ingestion of glucose and fructose during exercise in the heat. Journal of Applied Physiology, 100(3), 807-816.
Konishi, K., Kimura, T., Yuhaku, A., Kurihara, T., Fujimoto, M., Hamaoka, T., & Sanada, K. (2017). Mouth rinsing with a carbohydrate solution attenuates exercise-induced decline in executive function. Journal of the International Society of Sports Nutrition, 14(1), 45.
Lambert, G.P., Lang, J., Bull, A., Eckerson, J., Lanspa, S., & O’Brien, J. (2008). Fluid tolerance while running: effect of repeated trials. International Journal of Sports Medicine, 29(11), 878-882.
Luden, N.D., Saunders, M.J., D’Lugos, A.C., Pataky, M.W., Baur, D.A., Vining, C.B., & Schroer, A.B. (2016). Carbohydrate mouth rinsing enhances high intensity time trial performance following prolonged cycling. Nutrients, 8(9), 576.
Mamus, R.T., Dos Santos, M.G., Campbell, B., & Kreider, R. (2006). Biochemical effects of carbohydrate supplementation in a simulated competition of short terrestrial duathlon. Journal of the International Society of Sports Nutrition, 3(2), 1-6.
Pfeiffer, B., Stellingwerff, T., Hodgson, A.B., Randell, R., Pöttgen, K., Res, P., & Jeukendrup, A.E. (2012). Nutritional intake and gastrointestinal problems during competitive endurance events. Medicine & Science in Sports & Exercise, 44(2), 344-351.
Pivarnik, J.M., Marichal, C.J., Spillman, T., & Morrow, J.R. (1992). Menstrual cycle phase affects temperature regulation during endurance exercise. Journal of Applied Physiology, 72(2), 543–548.
Pottier, A., Bouckaert, J., Gilis, W., Roels, T., & Derave, W. (2010). Mouth rinse but not ingestion of a carbohydrate solution improves 1‐h cycle time trial performance. Scandinavian Journal of Medicine & Science in Sports, 20(1), 105-111.
Rollo, I., Cole, M., Miller, R., & Williams, C. (2010). Influence of mouth rinsing a carbohydrate solution on 1-h running performance. Medicine and Science in Sports and Exercise, 42(4), 798-804.
Rollo, I., Williams, C., Gant, N., & Nute, M. (2008). The influence of carbohydrate mouth rinse on self-selected speeds during a 30-min treadmill run. International Journal of Sport Nutrition and Exercise Metabolism, 18(6), 585-600.
Smith, M.J., Adams, L.F., Schmidt, P.J., Rubinow, D.R., & Wassermann, E.M. (2002). Effects of ovarian hormones on human cortical excitability. Annals of Neurology, 51(5), 599-603.
Tanaka, M., & Watanabe, Y. (2012). Supraspinal regulation of physical fatigue. Neuroscience and Biobehavioral Reviews. 36, 727-734.
Tenan, M.S., Hackney, A.C., & Griffin, L. (2016). Maximal force and tremor changes across the menstrual cycle. European Journal of Applied Physiology, 116(1), 153-160.
Thomas, T., Erdman, K.A., & Burke, L.M. (2016). American college of sports medicine, academy of nutrition and dietetics, and dietitians of Canada, joint position statement: nutrition and athletic performance. Medicine and Science in Sports and Exercise, 48(3), 543–568.
Van Cutsem, J., De Pauw, K., Buyse, L., Marcora, S.M., Meeusen, R., & Roelands, B. (2017). Effects of mental fatigue on endurance performance in the heat. Medicine & Science in Sports & Exercise, 49(8), 1677-1687.
van Melick, N., Meddeler, B.R., Hoogeboom, T.J., Nijhuis-van der Sanden, M.W,G., & van Cingel, R.E.H. (2017). How to determine leg dominance: The agreement between self-reported and observed performance in healthy adults. PloS One, 12(12), e0189876.