Document Type : Original Article
Authors
1 Associate Professor at Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran.
2 Ph.D in Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.
3 Associate Professor at Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Iran.
4 Professor of Department of Internal Medicine, University of Medical Sciences, Tabriz, Iran.
Abstract
Background and Aim: Gastrointestinal symptoms (increased intestinal damage, permeability & endotoxemia) are common and a limiting factor in acute, and prolonged strenuous exercises. Based on the ambiguous acute and chronic effects of high-intensity interval training (HIIT) on gastrointestinal function, this study was conducted to identify the effect of one-session and eight-week HIIT on some gastrointestinal syndrome markers in female athletes. Materials and Methods: Twenty athlete’s girls participated in a quasi-experimental design that were allocated into equal groups: the control group (n=10) and HIIT (n=10). The single-session sports protecol consisted of 18 times of running 400 meters with an intensity of 100 to 110% of the maximal oxygen consumption, which increased from nine to 18 repetitions of 400 meters during the training period (eight weeks). Blood samples were collected immediately before and two hours after exercise protocols. Serum intestinal fatty acid-binding protein (I-FABP), Zonulin, lipopolysaccharide (LPS), and immunoglobulin M (IgM) markers were assessed using ELISA method. Data was analyzed using repeated-measures analysis of variance and Wilcoxon at a significance level of p<0.05. Results: One-session acute HIIT induced a significant decrease in IgM, and increases in I-FABP, Zonulin and LPS concentrations at the beginning and end of the training period (p<0.05). However, the acute increased response in Zonulin at the beginning; and Zonulin and IgM at the end of the training period returned to baseline levels after two hours of recovery. The LPS and IgM concentrations differences between the control and HIIT groups were not significant after eight weeks of training (p>0.05). Conclusion: An acute HIIT session may increase permeability of I-FABP and gastrointestinal endotoxemia. However, the positive adaptations resulting from HIIT (eight weeks) can improve the exercise-induced response of intestinal integrity markers in female athletes.
Keywords
Barberio, M.D., Elmer, D.J., Laird, R.H., Lee, K.A., Gladden, B., & Pascoe, D.D. (2015). Systemic LPS and inflammatory response during consecutive days of exercise in heat. International Journal of Sports Medicine, 36(03), 262-270.
Bartlett, J.D., Close, G.L., MacLaren, D.P., Gregson, W., Drust, B., & Morton, J.P. (2011). High-intensity interval running is perceived to be more enjoyable than moderate-intensity continuous exercise: implications for exercise adherence. Journal of Sports Sciences, 29(6), 547-553.
Bressa, C., Bailén-Andrino, M., Pérez-Santiago, J., González-Soltero, R., Pérez, M., Montalvo-Lominchar, M.G., ... & Larrosa, M. (2017). Differences in gut microbiota profile between women with active lifestyle and sedentary women. Public Library of Science, 12(2), e0171352.
Costa, R.J., Miall, A., Khoo, A., Rauch, C., Snipe, R., Camões-Costa, V., & Gibson, P. (2017). Gut-training: The impact of two weeks repetitive gut-challenge during exercise on gastrointestinal status, glucose availability, fuel kinetics, and running performance. Applied Physiology, Nutrition, and Metabolism, 42(5), 547-557.
Costa, R.J.S., Snipe, R.M.J., Kitic, C.M., & Gibson, P.R. (2017). Systematic review: exercise‐induced gastrointestinal syndrome—implications for health and intestinal disease. Alimentary Pharmacology & Therapeutics, 46(3), 246-265.
Cunningham, J.J. (1991). Body composition as a determinant of energy expenditure: a synthetic review and a proposed general prediction equation. The American Journal of Clinical Nutrition, 54(6), 963-969.
De Oliveira, E.P., & Burini, R.C. (2009). The impact of physical exercise on the gastrointestinal tract. Current Opinion in Clinical Nutrition & Metabolic Care, 12(5), 538-533.
Dokladny, K., Zuhl, M.N., & Moseley, P.L. (2016). Intestinal epithelial barrier function and tight junction proteins with heat and exercise. Journal of Applied Physiology, 120(6), 692-701.
Edwards, K.H., Ahuja, K.D., Watson, G., Dowling, C., Musgrave, H., Reyes, J., ... & Kitic, C.M. (2021). The influence of exercise intensity and exercise mode on gastrointestinal damage. Applied Physiology, Nutrition, and Metabolism, 46(9), 1105-1110.
El Asmar, R., Panigrahi, P., Bamford, P., Berti, I., Not, T., Coppa, G.V., … & Fasano, A. (2002). Host-dependent zonulin secretion causes the impairment of the small intestine barrier function after bacterial exposure. Gastroenterology, 123(5), 11615-607
Ghosh, S.S., Wang, J., Yannie, P.J., & Ghosh, S.H. (2020). Intestinal barrier dysfunction, LPS translocation, and disease development. Journal of the Endocrine Society, 4(2), bvz039.
Hill, G.W., Gillum, T.L., Lee, B.J., Romano, P.A., Schall, Z.J., Hamilton, A.M., & Kuennen, M.R. (2020). Prolonged treadmill running in normobaric hypoxia causes gastrointestinal barrier permeability and elevates circulating levels of pro-and anti-inflammatory cytokines. Applied Physiology, Nutrition, and Metabolism, 45(4), 376-386.
Jin, S., Kim, S.K., Seo, H.J., Kim, M., Ahn, K.T., Kim, J.H., ... & Jeong, J. (2017). The curves exercise suppresses endotoxemia in korean women with obesity. Journal of Korean Medical Science, 32(2), 272-277.
Karhu, E., Forsgård, R.A., Alanko, L., Alfthan, H., Pussinen, P., Hämäläinen, E., & Korpela, R. (2017). Exercise and gastrointestinal symptoms: running-induced changes in intestinal permeability and markers of gastrointestinal function in asymptomatic and symptomatic runners. European Journal of Applied Physiology, 117(12), 2519-2526.
Keirns, B.H., Koemel, N.A., Sciarrillo, C.M., Anderson, K.L., & Emerson, S.R. (2020). Exercise and intestinal permeability: another form of exercise-induced hormesis? American Journal of Physiology-Gastrointestinal and Liver Physiology, 319(4), G512-G518.
Lamprecht, M., Bogner, S., Schippinger, G., Steinbauer, K., Fankhauser, F., Hallstroem, S., ... & Greilberger, J.F. (2012). Probiotic supplementation affects markers of intestinal barrier, oxidation, and inflammation in trained men; a randomized, double-blinded, placebo-controlled trial. Journal of the International Society of Sports Nutrition, 9(1), 1-13.
Lira, F.S., Rosa, J.C., Pimentel, G.D., Souza, H.A., Caperuto, E.C., Carnevali, L.C., ... & de Mello, M.T. (2010). Endotoxin levels correlate positively with a sedentary lifestyle and negatively with highly trained subjects. Lipids in Health and Disease, 9(1), 1-5.
March, D.S., Marchbank, T., Playford, R.J., Jones, A.W., Thatcher, R., & Davison, G. (2017). Intestinal fatty acid-binding protein and gut permeability responses to exercise. European Journal of Applied Physiology, 117(5), 931-941.
Motiani, K.K., Collado, M.C., Eskelinen, J., Virtanen, K.A., Löyttyniemi, E., Salminen, S., ... & Hannukainen, J.C. (2020). Exercise training modulates gut microbiota profile and improves endotoxemia. Medicine & Science in Sports & Exercise, 52(1), 94.
Ogden, H.B., Fallowfield, J.L., Child, R.B., Davison, G., Fleming, S.C., Edinburgh, R.M., ... & Layden, J.D. (2020). Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress. Physiological Reports, 8(5), e14374.
Pasini, E., Corsetti, G., Assanelli, D., Testa, C., Romano, C., Dioguardi, F.S., & Aquilani, R. (2019). Effects of chronic exercise on gut microbiota and intestinal barrier in human with type 2 diabetes. Minerva Medica, 110(1), 3-11.
Pfeiffer, B., Cotterill, A., Grathwohl, D., Stellingwerff, T., & Jeukendrup, A.E. (2009). The effect of carbohydrate gels on gastrointestinal tolerance during a 16-km run. International Journal of Sport Nutrition and Exercise Metabolism, 19(5), 485-503.
Pugh, J.N., Impey, S.G., Doran, D.A., Fleming, S.C., Morton, J.P., & Close, G.L. (2017). Acute high-intensity interval running increases markers of gastrointestinal damage and permeability but not gastrointestinal symptoms. Applied Physiology, Nutrition, and Metabolism, 42(9), 941-947.
Riebe, D., Ehrman, J.K., Liguori, G., Magal, M. (2018). ACSM’s guidelines for exercise testing and prescription. 10th Edition. American College of Sports Medicine.
Sasaki, M., & Joh, T. (2007). Oxidative stress and ischemia-reperfusion injury in gastrointestinal tract and antioxidant, protective agents. Journal of Clinical Biochemistry and Nutrition, 40(1), 1-12.
Seiler, S. (2010). What is best practice for training intensity and duration distribution in endurance athletes? International Journal of Sports Physiology and Performance, 5(3), 276-291.
Snipe, R.M.J., & Costa, R.J.S. (2018). Does biological sex impact intestinal epithelial injury, small intestine permeability, gastrointestinal symptoms and systemic cytokine profile in response to exertional-heat stress? Journal of Sports Sciences, 36(24), 2827-2835.
Snipe, R.M.J., Khoo, A., Kitic, C.M., Gibson, P.R., & Costa, R.J.S. (2017). Carbohydrate and protein intake during exertional heat stress ameliorates intestinal epithelial injury and small intestine permeability. Applied Physiology, Nutrition, and Metabolism, 42(12), 1283-1292.
Snipe, R.M.J., Khoo, A., Kitic, C.M., Gibson, P.R., & Costa, R.J.S. (2018). The impact of mild heat stress during prolonged running on gastrointestinal integrity, gastrointestinal symptoms, systemic endotoxin and cytokine profiles. International Journal of Sports Medicine, 39(04), 255-263.
Tota, L., Piotrowska, A., Palka, T., Morawska, M., Mikuiáková, W., Mucha, D., & ... Pilch, W. (2019). Muscle and intestinal damage in triathletes. PLos One, 14(1), e0210651.
Van Wijck, K., Lenaerts, K., Van Loon, L.J.C., Peters, W.H.M., Buurman, W.A., & Dejong, C.H.C. (2011). Exercise-induced splanchnic hypoperfusion results in gut dysfunction in healthy men. PloS One, 6(7), e22366.
van Wijck, K., Pennings, B., van Bijnen, A.A., Senden, J.M.G., Buurman, W.A., Dejong, C.H.C., ... & Lenaerts, K. (2013). Dietary protein digestion and absorption are impaired during acute postexercise recovery in young men. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 304(5), R356-R361.
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