Document Type : Original Article
Authors
1 1. Associate Professor in Exercise Physiology, Department of Exercise Physiology, School of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran.
2 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Iran.
3 3. Ph.D. student, Department of Exercise Physiology, School of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
Abstract
Background and Aim: Micro-RNAs (miRNA) are short RNA molecules that as significant regulators of physiological processes associated with obesity play important roles in body; this is while the combination of effect of interval and continuous aerobic training along with calorie restriction can affect the gene expression of miR-146a and some inflammatory factors like tumor necrosis factor- α (TNF-α), interleukin -6 (IL-6) and C-reactive protein (CRP). Therefore, the aim of the present study was to determine the effect of two types of exercise training along with intermittent calorie restriction (ICR) on miR-146a expression and TNF-α, IL-6 and CRP levels in obese women. Materials and Methods: Twenty-eight women were selected by targeted sampling from obese women aged 35-45 years in Tabriz city and were divided randomly into four groups including of ICR (ICR, 20 percent), high intensity interval training and ICR (10 percent calorie restriction from exercise and 10 percent ICR), a combination of continuous aerobic training and ICR (10 percent calorie restriction from exercise and 10 percent ICR) and control groups. Interval training (up to 75-95% of the maximum reserve heart rate) and continuous training (up to 60-75% of the maximum reserve heart rate) was performed for 8 weeks with repetition of 3 sessions per week. Dependent variables were measured before the intervention and 72 hours after the last training session. Results were extracted using Shapiro-Wilk, repeated measure analysis of variance and Bonferroni tests at the significance level of p<0.05. Findings: Both training groups with calorie restriction compared to the control group showed a significant increase in maximal oxygen uptake and miR-146a expression and a significant decrease in TNF-α, IL-6 and CRP (p<0.05). While, there was no significant difference between the two groups of interval and continuous aerobic exercise with calorie restriction (p<0.05.) Conclusion: Doing exercise (regardless of whether it is interval or continuous) and limiting daily calorie intake can be effective in reducing chronic inflammation in obese people and can be used as a non-pharmacological method to help solve the problems of these people.
Keywords
Abedi, B. (2012). The effects of 12-wk combined aerobic/resistance training on C-reactive protein (CRP) serum and interleukin-6 (IL-6) plasma in sedentary men. Yafteh,14(4), 95-106. [Persian]
Adams, O. P. (2013). The impact of brief high-intensity exercise on blood glucose levels. Diabetes, metabolic syndrome and obesity. targets and therapy, 6, 113.
Allen, N. G., Higham, S. M., Mendham, A. E., Kastelein, T. E., Larsen, P. S., & Duffield. R. ( 2017). The effect of high-intensity aerobic interval training on markers of systemic inflammation in sedentary populations. European Journal of Applied Physiology, 117(6), 1249-56.
Bahram, M. E., Najjarian, M., Sayyah, M., & Mojtahedi, H. (2013). The effect of an eight-week aerobic exercise program on the homocysteine level and VO2max in young non-athlete men. Feyz, Journal of Kashan University of Medical Sciences, 17(2), 149-156.
Barzegar, H., Vosadi, E., & Borjian Fard, M. (2017). The effect of different modes of training on plasma levels of IL-6 and IL-10 in male. Journal of Sport Biosciences, 9( 2), 171-181. [Persian]
Barnosky ,A. R., Hoddy, K. K., Unterman, T. G ., & Varady, K. A. (2014). Intermittent fasting vs daily calorie restriction for type 2 diabetes prevention.Translational Research, 164(4), 302-11.
Blondal, T., Nielsen, S. J., Baker, A., Andreasen, D., Mouritzen, P., Teilum, M. W., & Dahlsveen, I. K. (2013). Assessing sample and miRNA profile quality in serum and plasma or other biofluids. Methods, 59(1), S1-S6.
Craenenbroeck, A. H., Ledeganck, K. J., VanAckeren, K., Jürgens, A., Hoymans, V. Y., Fransen, E., ... & Van Craenenbroeck, E. M. (2015). Plasma levels of microRNA in chronic kidney disease patterns in acute and chronic exercise, American Journal of Physiology-Heart and Circulatory Physiology, 309(12), H2008-H16.
Davis, R. A., Halbrooks, J. E., Watkins, E. E., Fisher, G., Hunter, G. R., Nagy, T. R., & Plaisance ,E. P. (2017). High-intensity interval training and calorie restriction promote remodeling of glucose and lipid metabolism in diet-induced obesity. American Journal of Physiology-Endocrinology and Metabolism, 313(2), 243-256.
Dev, N., & Marcus, S. R., (2012). High sensitive C-reactive protein, an independent and early novel inflammatory marker in healthy obese women. Biomedical Research, 23(1), 73-77.
Falah, F., Rahmani Nia, F., Shabani, R., & Hojati, Z. (2019). Investigation on the effect of endurance training on blood circulating levels of Mir-146a and plasma levels of il-6 in sedentary elderly women. Journal of Shahid Sadoughi University of Medical Sciences, 26(10), 895-909. [Persian]
Ferguson, B. (2014). ACSM’s Guidelines for Exercise Testing and Prescription 9th Ed. The Journal of the Canadian Chiroractic Association. 58(3), 328.
Foster, V., Farland, C. V., Guidotti, F., Harbin, M., Roberts, B., Schuette, J., Tuuri, A., Doberstein, S. T., & Porcari, J. P. (2015). The effects of high intensity interval training vs steady state training on aerobic and anaerobic capacity. Journal of Sports Science & Medicine, 14(4), 747–755.
Gerosa-Neto, J., Antunes, B., Campos, E., Rodrigues, J., Ferrari, G. D., Neto, J. C., Bueno, C. R., & Lira, L. S. (2016). Impact of lon g-term high-intensity interval and moderate intensity continuous training on subclinical inflammation in overweight/obese adults. Journal of Exercise Rehabilitation, 12(6), 575-580.
Goossens, GH. (2008). The role of adipose tissue dysfunction in the pathogenesis of obesity-related insulin resistance. Physiology & Behavior, 94(2), 206-18.
Goyal, R., Faizy, AF., Siddiqui, S. S., & Singhai, M. (2012). Evaluation of TNF-α and IL-6 levels in obese and non-obese diabetics: pre-and postinsulin effects. North American Journal of Medical Sciences, 4(4), 180.
Gibson, A. L., Wagner, D., & Heyward, V. (2018). Advanced Fitness Assessment and Exercise Prescription. 8nd Edition, Human kinetics.
Gibson, R. S. (2005). Principles of nutritional assessment. 2nd Edition. New York: Oxford University Press; 149-96.
Huszar, J., & Payne, C. (2013). miR-146a Influences energy metabolism. Cell Differentiation and Innate Immunity Metabolomics, 3(1), 119.
Imayama, I., Ulrich, C. M., Alfano, C. M., Wang, C., Xiao, L., Wener, M. H., … & Tiernan, A. M. (2012). Effects of a caloric restriction weight loss diet and exerciseon inflammatory biomarkers in overweight/obese postmenopausal women. Cancer Research, 72(9), 2314-26.
Improta Caria, A. C., Nonaka, C. K. V., Pereira, C. S., Soares, M. B. P., Macambira, S. G., & Souza, B. S. d. (2018). Exercise training-induced changes in micrornas. beneficial regulatory effects in hypertension, type 2 diabetes, and obesity. International Journal of Molecular Sciences, 19(11), 3608.
Johnstone, A. (2015). Fasting for weight loss: an effective strategy orlatest dieting trend? International Journal of Obesity, 39, 727-733.
Kangas, R., Tormakangas, T., Heinonen, A., Alen, M., Suominen, H., Kovanen, V., … & Korhonen , M. T. (2017). Declining physical performance associates with serum FasL, miR-21, and miR-146a in aging. Sprinters BioMed Research International, 2017, 1-14.
Kiyosawa, N., Watanabe, K., Toyama, K., & Ishizuka, H. (2019). Circulating miRNA signature as a potential biomarker for the prediction of analgesic efficacy of hydromorphone. Journal of Molecular Sciences, 20(7), 1665.
Longo, M., Zatterale, F., Naderi, J., Parrillo, L., Formisano, P., Raciti, G, A., Beguinot, F., & Miele, C. (2019). Review adipose tissue dysfunction as determinant of obesity associated metabolic complications. International Journal of Molecular Sciences, 20(9), 2358.
Lopes, W. A., Leite, N, d., Silva, L. R., Brunelli, D. T., Gáspari, A. F., Radominski, R. B., … & Cavaglieri, C. R. (2016). Effects of 12 weeks of combined trainingwithout caloric restriction on inflammatory markers in overweight girls. Journal of Sports Science, 34(20),1902.
Mohaad zadeh, M. A., Kargarfard, M., & Nazar, A. (2019). Comparison of the ffects of interval training with different diet on IL-6, TNF-α and some of metabolicindexes in obesity type 2 ediabetic men. Iranian Journal of Diabetes and Metabolism, 18(3), 156-172. [Persian]
Nikroo, H., & Barancheshme, M. A. (2014). The comparison of effects of aerobic interval and continuous training program on maximal oxygen consumption, body mass index and body fat percentage in officer students. Journal of Military Medicine, 15(4), 245-251.
Oghbaei, H., Ahmadi Asl, N., & Sheikhzadeh, F. (2017). Can regular moderate exercise lead to changes in miRNA-146a and its adapter proteins in the kidney of streptozotocin-induced diabetic male rats? Endocrine Regulations, 51(3), 145-52. [Persian]
Olivieri, F., Rippo, M. R., Prattichizzo, F., Babini, L., Graciotti, L., Recchioni, R., & Procopio, A. D. (2013). Toll like receptor signaling in “inflammaging”. microRNA as new players. Immunity & Ageing, 10(1), 11.
Qiulian, Z., Chao, S., Yicheng, L., Chenglin, Z., Zheng, J., & Tianhui, W. (2020). Circulating microRNAs in response to exercise training in healthy adults, Frontiers In Genetics, 11, 256.
Davis, R. A., Halbrooks, J. E., Watkins, E. E., Fisher, G., Hunter, G. R., Nagy, T. R., & Plaisance, E. P. (2017). High-intensity interval training and calorie restriction promote remodeling of glucose and lipid metabolism in diet-induced obesity. American Journal of Physiology-Endocrinology and Metabolism, 313(2), E243-E256.
Rahimi, N., Bambaiechi, E., & Reisi, J. (2017). Comparison the effect of eight weeks high intensity interval and moderate continuous trainings on pain reductionand and blood levels of IL6 and TNF-α in woman with primary dysmenorrhea. Research in Medicine, 41(4), 258-268. [Persian]
Rajabi, H., & Ghaeini, A. A. (2004). Physical Fitness. 2th Edition. Tehran: Samt. 68-107. [Persian]
Reed, J. L., De Souza, M. J., & Williams, N. I. (2010). Effects of exercise combined with caloric restriction on inflammatory cytokines. Applied Physiology, Nutrition and Metabolism, 35(5), 573-82.
Russo, A., Bartolini, D., Mensà, E., Torquato, P., Albertini, M. C., Olivieri, F., … & Galli, F. (2018). Physical activity modulates the overexpression of the inflammatory miR-146a-5p in obese patients. International Union of Biochemistry and Molecular Biology Life, 70(10), 1012-1022.
Sapp, R. M., & Hagberg, J. M. (2019). Circulating microRNAs: advances in exercise physiology. Current Opinion Physiology, 10, 1-9.
Sawada, S., Kon, M., Wada, S., Ushida, T., Suzuki, K., & Akimoto, T. (2013). Profiling of circulating microRNAs after a bout of acute resistance exercise in humans. PloS One, 8(7), e70823.
Siahkouhian, M., & Khodadadi, D. (2013). The effect of high intensity interval training and moretete- intensity comtinuous training on aerobic and anaerobic indices in athlete boys. Sports Physiology (Research on sport science), 5(18), 39. [Persian]
e Silva, A. D. S., & da Mota, M. P. G. (2014). Effects of physical activity and training programs on plasma homocysteine levels: a systematic review. Amino Acids, 46(8), 1795-1804.
Sirotkin, A. V., Ovcharenko, D., Grossmann, R., Lauková, M., & Mlyncek, M. (2009). Identification of MicroRNAs controlling human ovarian cell steroidogenesis via a genome-scale screen. Journal of Cellular Physiology, 219(2), 415-420.
Sharifzadeh, S., & Keshavarz, Z. (2015). Diagnostic value of cell free fetal DNA for detection of fetal abnormalities. Journal of Laboratory and Diagnosis. 12(47), 8-22. [Persian]
Varady, K. A. (2011). Intermittent versus daily calorie restriction: Which diet regimen is more effective for weight loss? Obesity Reviws, 12(7), 593-601.
Verit, F. F., Yildiz Zeyrek, F., Zebitay, A. G., & Akyol, H. (2017). Cardiovascular risk may be increased in womenwith unexplained infertility. Clinical and Exprimental Reproductive Medicine, 44(1), 28-32.
Yamaner, F., Bayraktaroglu, T., Atmaca, H., Ziyagil, M. A., & Tamer, K. ( 2010). Serum leptin, lipoprotein levels, and glucose homeostasis between national wrestlers and sedentary males. Turkish Journal of Medical Sciences, 40(3), 471-477.
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