Document Type : Original Article
Authors
- Gabrial Pouzesh Jadidi 1
- Farnaz Seifi-Skishahr 2
- Lotfali Bolboli 2
- Karim Azali Alamdari 3
- Amenh Pourrahim Ghouroghch 4
1 Ph.D Student of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Associate Professor at Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
3 Associate Professor at Department of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran.
4 Assistant Professor at Department of Exercise Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
Abstract
Background and Aim: Imbalance of some microRNAs and its cell apoptosis has an important role in myocardial infarction. Based on the role of curcumin supplementation and high-intensity interval training (HIIT) on myocardial infarction, the aim of this study was to investigate the effects of these types of training and supplementation on left ventricular gene expression levels of miR-133 and miR-1 in the isoproterenol-induced myocardial infarction (MI) rat model. Materials and Methods: Forty male Wistar rats were randomly divided into four groups as: HIIT, curcumin, HIIT+curcumin and control groups, following induction of MI via intraperitoneal injection of isopretrenol (100 mg/kg/day) within two consecutive days. The HIIT protocol was performed during eight weeks (five sessions per week), each session was consisted of 10 bouts of running occasions (each for four min) at 85 - 90% of vVo2max with two min rest intervals at 50 to 60% of vVO2max. Moreover, Curcumin (15 mg/kg) was administered daily via gavage. The gene expression levels of miR-1 and miR-133 were evaluated using Real-Time PCR method and the data were analyzed using one-way analysis of variance and Tukey post hoc test at the significance level of p<0.05. Results: Although, in all three intervention groups of HIIT, curcumin and HIIT+curcumin, the cardiomyocytes miR-1 gene expression level was significantly lower (p=0.001), while miR-133 expression level was higher (p=0.001) than control group. However, gene expression in both variables after HIIT+curcumin showed more changes than curcumin group (p=0.006 and p=0.01 respectively). Conclusion: Probably, curcumin supplementation along with HIIT training after myocardial infarction is associated with improvement of cardiac function; but due to research limitations, more research is needed.
Keywords
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