Document Type : Original Article
Authors
1 MSc in Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
2 Full Professor, Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
Abstract
Background and Aim: Since many cellular and molecular pathways involve in diabetes are unknown, the aim of the present study was to study the effects of aerobic exercise on glycogen stores in pre-diabetic rats. Materials and Methods: Twenty-one homogenous male C57BL/6 mice were randomly divided into two groups including Mice fed standard diet (n=7) and Mice fed high fat diet (n=14). The mice were fed for 12 weeks and then the pre-diabetic conditions of the mice were assessed by fasting glucose and glucose tolerance tests. After confirmation of pre-diabetic conditions, mice fed a high-fat diet were divided into two groups as high-fat-sedentary diet, and high-fat diet-aerobic exercise. Mice in the training group were trained on a treadmill for 10 weeks, five days a week for 45 minutes in every session. Twenty-four hours after the last training session, the rats were killed and blood was drawn from the rats’ hearts. Liver tissue was isolated and frozen to measure glycogen synthase gene expression (RT-Real time PCR) in liquid nitrogen. The independent t-test and one-way analysis of variance were used for statistical analysis at the p<0.05 level. Results: The glycogen synthase levels in the aerobic exercise group were higher than the prediabetes group and had a significant effect on glycogen synthase expression (p=0.001). Also, liver glycogen showed significantly increase (p=0.001) in the amount of hepatic glycogen stores in the exercise group compared to the prediabetes group. Conclusion: Aerobic exercise can lead to the improvement of the condition from pre-diabetic to healthy condition.
Keywords
Cheong, J.L. K., Croft, K., Henry, P., Matthews, V., Hodgson, J., & Ward, N. (2014). Green coffee polyphenols do not attenuate features of the metabolic syndrome and improve endothelial function in mice fed a high fat diet. Archives of Biochemistry and Biophysics, 559, 46-52.
Delevatti, R.S., Bracht, C.G., Lisboa, S.D.C., Costa, R.R., Marson, E.C., Netto, N., & Kruel, L.F.M. (2019). The role of aerobic training variables progression on glycemic control of patients with Type 2 Diabetes: a systematic review with meta-analysis. Sports Medicine-open, 5(1), 1-17.
Edgerton, D.S., Johnson, K., & Cherrington, A.D. (2009). Current strategies for the inhibition of hepatic glucose production in type 2 diabetes. Frontiers in Bioscience, 14, 1169-1181.
Hordern, M.D., Dunstan, D.W., Prins, J.B., Baker, M.K., Singh, M.A.F., & Coombes, J.S. (2012). Exercise prescription for patients with type 2 diabetes and pre-diabetes: a position statement from exercise and sport science Australia. Journal of Science and Medicine in Sport, 15(1), 25-31.
Hou, D., Zhao, Q., Yousaf, L., Chen, B., Xue, Y., & Shen, Q. (2020). A comparison between whole mung bean and decorticated mung bean: beneficial effects on the regulation of serum glucose and lipid disorders and gut microbiota in high-fat diet and streptozotocin-induced prediabetic mice. Food & Function, 11(6), 5525-37.
Irimia, J.M., Meyer, C.M., Peper, C.L., Zhai, L., Bock, C.B., Previs, S.F., . . . & Roach, P.J. (2010). Impaired glucose tolerance and predisposition to the fasted state in liver glycogen synthase knock-out mice. Journal of Biological Chemistry, 285(17), 12851-12861.
Jafari, T., Mahmoodnia, L., Tahmasebi, P., Memarzadeh, M.R., Sedehi, M., Beigi, M., & Fallah, A.A. (2018). Effect of cumin (Cuminum cyminum) essential oil supplementation on metabolic profile and serum leptin in pre-diabetic subjects: A randomized double-blind placebo-controlled clinical trial. Journal of Functional Foods, 47, 416-422.
Jeong, S., Cho, J. M., Kwon, Y.-I., Kim, S.-C., Shin, D. Y., & Lee, J. H. (2019). Chitosan oligosaccharide (GO2KA1) improves postprandial glycemic response in subjects with impaired glucose tolerance and impaired fasting glucose and in healthy subjects: a crossover, randomized controlled trial. Nutrition & Diabetes, 9(1), 1-9.
Karthikesan, K., Pari, L., & Menon, V.P. (2010). Protective effect of tetrahydrocurcumin and chlorogenic acid against streptozotocin–nicotinamide generated oxidative stress induced diabetes. Journal of Functional Foods, 2(2), 134-142.
Kazeminasab, F., Marandi, S.M., Ghaedi, K., Safaeinejad, Z., Esfarjani, F., & Nasr-Esfahani, M.H. (2018). A comparative study on the effects of high-fat diet and endurance training on the PGC-1α-FNDC5/irisin pathway in obese and nonobese male C57BL/6 mice. Applied Physiology, Nutrition, and Metabolism, 43(7), 651-662.
Khan, R.M.M., Chua, Z.J.Y., Tan, J.C., Yang, Y., Liao, Z., & Zhao, Y. (2019). From pre-diabetes to diabetes: diagnosis, treatments and translational research. Medicina, 55(9), 546.
Kreft, H., & Jetz, W. (2007). Global patterns and determinants of vascular plant diversity. Proceedings of the National Academy of Sciences, 104(14), 5925-5930.
Kumar, A.S., Maiya, A.G., Shastry, B., Vaishali, K., Ravishankar, N., Hazari, A., . . . & Jadhav, R. (2019). Exercise and insulin resistance in type 2 diabetes mellitus: A systematic review and meta-analysis. Annals of Physical and Rehabilitation Medicine, 62(2), 98-103.
Ma, Y., Gao, M., & Liu, D. (2015). Chlorogenic acid improves high fat diet-induced hepatic steatosis and insulin resistance in mice. Pharmaceutical Research, 32(4), 1200-1209.
MacAulay, K., & Woodgett, J.R. (2008). Targeting glycogen synthase kinase-3 (GSK-3) in the treatment of type 2 diabetes. Expert Opinion on Therapeutic Targets, 12(10), 1265-1274.
McCarty, M.F. (2005). A chlorogenic acid-induced increase in GLP-1 production may mediate the impact of heavy coffee consumption on diabetes risk. Medical Hypotheses, 64(4), 848-853.
Parveen, S., & Khan, A.A. (2020). An Insight of Prediabetes. Journal of Pharmaceutical Research, 1(01), 02-08.
Petersen, M.C., & Shulman, G.I. (2018). Mechanisms of insulin action and insulin resistance. Physiological Reviews, 98(4), 2133-2223.
Sarani Maram, Y., Vahidian-Rezazadeh, M., & Fanaei, H. (2020). The interactive effects of resistance, endurance trainings and consumption of nettle extract on plasma apelin levels and weight changes in diabetic type 1 rats. Journal of Practical Studies of Biosciences in Sport, 8(16), 72-84. [Persian]
Song, S.J., Choi, S., & Park, T. (2014). Decaffeinated green coffee bean extract attenuates diet-induced obesity and insulin resistance in mice. Evidence-based Complementary and Alternative Medicine, 2014, 718379.
Van Dijk, A.E., Olthof, M.R., Meeuse, J.C., Seebus, E., Heine, R.J., & Van Dam, R.M. (2009). Acute effects of decaffeinated coffee and the major coffee components chlorogenic acid and trigonelline on glucose tolerance. Diabetes Care, 32(6), 1023-1025.
Venables, M.C., & Jeukendrup, A.E. (2008). Endurance training and obesity: effect on substrate metabolism and insulin sensitivity. Medicine and Science in Sports and Exercise, 40(3), 495-502.
Wang, S., Ma, W., Yuan, Z., Wang, S.-m., Yi, X., Jia, H., & Xue, F. (2016). Association between obesity indices and type 2 diabetes mellitus among middle-aged and elderly people in Jinan, China: a cross-sectional study. BMJ open, 6(11), e012742.
Xu, D., Jiang, Z., Sun, Z., Wang, L., Zhao, G., Hassan, H. M., . . . & Zhang, L. (2019). Mitochondrial dysfunction and inhibition of myoblast differentiation in mice with high‐fat‐diet‐induced pre‐diabetes. Journal of Cellular Physiology, 234(5), 7510-7523.
Xu, L., Lin, X., Guan, M., Zeng, Y., & Liu, Y. (2019). Verapamil attenuated prediabetic neuropathy in high-fat diet-fed mice through inhibiting TXNIP-mediated apoptosis and inflammation. Oxidative Medicine and Cellular Longevity, 2019.
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