Document Type : Original Article
Authors
1 PhD Student in Exercise Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Professor of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Instructor of Department of Exercise Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
4 PhD Student in Exercise Physiology, Faculty of Sports Sciences, University of Birjand, Birjand, Iran.
Abstract
Background and Aim: The effect of occlusion training at different time intervals on muscle growth factors in girls is a new topic. Therefore, the present study conducted to study effects of resistance training with and without vascular occlusion on serum levels of some anabolic and catabolic hormones in active girls. Materials and Methods: For this purpose, 36 female students of physical education with an average body mass index of 23.32±2.79 kg/m2 were divided into three groups (n=12) including resistance training without vascular occlusion, resistance training with vascular occlusion, and control groups. Exercise groups performed resistance training for 30 minutes, three sessions per week, for six weeks and vascular occlusion was applied by closing the tourniquet around the proximal arm with a pressure of 100 mmHg. Fasting blood samples were collected before the start, 24 hours after, the 6th session, and the 18th session of the exercise protocol. Serum levels of insulin-like growth factor-1, growth hormone, cortisol, testosterone, and insulin were measured by Elisa method. To extract results, the analysis of variance with repeated measures and Bonferroni post hoc tests were applied at the significant level of p<0.05. Results: The results showed that after six weeks of intervention, the occlusion and non-occlusion resistance training reduced significantly both cortisol (p=0.01) and insulin (p=0.01) hormones; while testosterone (p=0.95) and insulin-like growth factor-1 (p=0.82) did not change significantly. Conclusion: The results showed that low-intensity occlusion resistance training can induce alterations in the insulin and cortisol hormones similar to high-intensity non-occlusion resistnace intervention.
Keywords
Ahtiainen, J .P., Pakarinen, A., Alen, M., Kraemer, W. J., & Häkkinen, K. (2005). Long restperiod between the sets in hypertrophic resistance training: Influence on muscle strength, size, and hormonal adaptations in trained men. The Journal of Strength & Conditioning Research, 19(3), 572-52.
Anthony, T. G., Anthony, J. C., Lewitt, M. S., Donovan, S. M., & Layman, DK. (2001). Time course changes in IGFBP1 after treadmill exercise and postexercise food intake in rats. American Journal of Physiology Endocrinology and Metabolism, 280, E650- E656.
Bruce, R. A., Kusumi, F., & Hosmer, D. (1973). Maximal oxygen intake and nomographic assessment of functional aerobic impairment in cardiovascular disease. American Heart Journal, 85, 548-582.
Brzycki, M. (1998). A practical approach to strength training. McGraw-Hill. Paperback, 3rd Edition, 249 pages.
Cappon, J., Brasel, J. A., Mohan, S., & Cooper, D. M. (1994). Effect of brief exercise on circulating insulin-like growth factor-1. Journal of Applied Physiology, 76(6), 1418-22.
Dabidi Roshan, V., Sadeghpour, G., & Jahanyan, Z. (2010). The effect of a debilitating session with different intensities on adiponectin levels in active women. Journal of Sports Life Sciences, 1(3), 21-39.
De Souza, T. M. F., Libardi, C. A., Cavaglieri, C. R., Gáspari, A. F., Brunelli, D. T., de Souza, G. V., ... & Chacon-Mikahil, M. P. T. (2018). Concurrent training with blood flow restriction does not decrease inflammatory markers. International Journal of Sports Medicine, 40(01), 29-36.
Fahs, C. A., Loenneke, J. P., Rossow, L. M., Tiebaud, R. S., & Bemben, M. G. (2012). Methodological considerations for blood flow restricted resistance exercise. Journal of Trainology, 1(1), 14–22.
Ghafari, G., Bolboli, L., Rajabi, A., & Saedmochshi, S. (2016). The effect of 8 weeks of aerobic exercise on inflammatory markers predicting atherosclerosis and lipid profile in obese elderly women. Journal of Ilam University of Medical Sciences, 23 (7), 144-154. [Persian]
Ghasemi, E., Afzalpour, M. E., Saghebjoo, M., & Zarban, A. (2012). Effects of short-term green tea supplementation on total antioxidant capacity and lipid peroxidation in young women after a resistance training session. Journal of Isfahan Medical School, 30(202), 1-10.
Godfrey, R. J., Madgwick, Z., & Whyte, G. P. (2003). The exercise-induced growth hormone response in athletes. Sports Medicine, 33(8), 599-613.
Goodman, H. M. (2003). Basic medical endocrinology. 3rd Edition. Amsterdam: Academic Press; 332-50.
Goto, K., Ishii, N., & Takamatsa, K. (2004). Growth hormone responseto training regimen with combined high and Lowintensityexercise. International Journal of Sport and Health Science, 2, 111-8.
Hosseini Kakhk, S. A., Zamand, P., Haghighi, A. H., & Khademsharie, M. (2015). Comparison of hormonal responses to strength training with and without blood flow restriction. Journal of Sports Life Sciences, 7(3), 391-405.
Hulmi, J. (2012). Molecular and hormonal responses and adaptation toresistance exercise and protein nutrition in young and older men. Academic dissertation by permission of the faculty of Sport and Health Science of the University of Jyvaskyla.
Jennische, E., & Hansson, H. A. (1987). Regenerating skeletal muscle cells express insulin-likegrowth factor I. Acta Physiologica Scandinavica, 130, 327-332.
Kahn, C. R. (1985). The molecular mechanism of insulin action. Annual Review of Medicine, 36(1), 429-451.
Kraemer, W. J., Harman, F. S., Vos, N. H., Gordon, S. E., Nindl, B. C., Marx, J. O., ... & Häkkinen, K. (2000). Effects of exercise and alkalosis on serum insulin-like growth factor I and IGF-binding protein-3. Canadian Journal of Applied Physiology, 25(2), 127-138.
Kraemer, W. J., & Ratamess, N. A. (2005). Hormonal responses and adaptations to resistance exercise and training. Sports Medicine, 35(4), 339-61.
Kurina, L. M., Weiss, L. A., Graves, S. W., Parry, R., Williams, G. H., Abney, M., & Ober, C. (2005). Sex differences in the genetic basis of morning serum cortisol levels: genome-wide screen identifies two novel loci specific to women. The Journal of Clinical Endocrinology & Metabolism, 90(8), 4747-4752.
Li, J., King, N. C., & Sinoway, L. I. (2003). ATP concentrations and muscle tension increaselinearly with muscle contraction. Journal of Applied Physiology, 95, 577-83.
Loenneke, J. P., Fahs, C. A., Rossow, L. M., Sherk, V. D., Thiebaud, R. S., Abe, T., ... & Bemben, M. G. (2012). Effects of cuff width on arterial occlusion: implications for blood flow restricted exercise. European Journal of Applied Physiology, 112(8), 2903-2912.
Manetta, J., Brun, J. F., Maimoun, L., Fedou, C., Prefaut, C., & Mercier, J. (2003). The effects ofintensive training on insulin- like growth factorI (IGF-1) and IGF binding proteins 1 and 3 in Competitive Cyclists: relationships with glucosedisposal. Journal of Sports Sciences, 21, 147-154.
Mohammadi, S., Madizadeh, R., Khoshdel, A. R., & Mirzaii-Dizgah. (2014). The effect of blood flow restricted resistance training on serum hormone levels in relation to muscle size and strength in young men. Ebnesina Journal, 45(4), 10-16. [Persian]
Moore, D. R., Burgomaster, K. A., Schofield, L. M., Gibala, M. J., Sale, D. G., & Phillips, S. M. (2004). Neuromuscular adaptations in human muscle following low intensity resistance training with vascular occlusion. European Journal of Applied Physiology, 92, 399-406.
Nakajima, T., Kurano, M., Iida, H., Takano, H., Oonuma, H., Morita, T., ... & Nagata, T. (2006). Use and safety of KAATSU training: results of a national survey. International Journal of KAATSU Training Research, 2(1), 5-13.
Naserkhani, F. (2015). Effect of one exercise session on a treadmill with limited blood flow on serum levels of growth hormones, insulin-like growth factor 1 and cortisol in female students-inactive. Master Thesis. Department of Physical Education, Faculty of Physical Education and Sports Sciences. Shahroud University. [Persian]
Porsesh, M., Habibi, A. H., Ahnadi Barati, S., & Fatemi Tabatabaei, R. (2016). Compare the effect of 6 weeks resistance training with and without vascular occlusion, on serum levels of CRP and LDH in active girls. Journal of Shahid Sadoughi University of Medical Sciences, 24(9), 706-715. [Persian]
Porsesh, M., Habibi, A. H., Ahnadi Barati, S., & Mardaniyan Ghahfarokhi, M. (2018). Comparison of the effect of a selected resistance exercise course with two different intensities on some metabolic indices in active young girls. Jundishapur Journal of Health Sciences, 17(2), 115-130. [Persian]
Rajabi, H., Soleymani Far, E., Hasani Ranjbar, S., & Heshmat, R. (2011). Monitoring of time course response in GH, Insulin, IGF-1, IGFBP-1 and IGFBP-3 after heavy resistance exercise in trained and non-trained men. Journal de Mathématiques Pures ET Appliquées, 1(1), 61-78.
Reeves, G. V., Kraemer, R. R., Hollander, D. B., Clavier, J., Thomas, C., Francois, M., & Castracane, V. D. (2006). Comparison of hormone responses following light resistance exercise with partial vascular occlusion and moderately difficult resistance exercise without occlusion. Journal of Applied Physiology, 101(6), 1616-1622.
Sedghi, B., Kahrizi, S., Zakeri, H. R., Omidfar, K., Rahmani, M. (2009). Evaluation of acute hormonal responses to concentric, eccentric and concentric muscle actions in healthy young men. Canadian Journal of Physiology and Pharmacology, 13(2), 216-28.
Seo, D. I., So, W. Y., Sung, D. J. (2016). Effect of a low-intensity resistance exercise program me with blood flow restriction on growth hormone and insulin-like growth factor-1 levels in middle-aged women. South African Journal for Research in Sport, Physical, 38(2), 167-77.
Silva, T. S., Longui, C. A., Faria, C. D. C., Rocha, M. N., Melo, M. R., Faria, T. G., ... & Kater, C. E. (2008). Impact of prolonged physical training on the pituitary glucocorticoid sensitivity determined by very low dose intravenous dexamethasone suppression test. Hormone and Metabolic Research, 40(10), 718-721.
Taghavi, M. R. (2008). Standardization of general health questionnaire on the students of Shiraz University (GHQ). Scientific- Research Journal of Shahed University Fifteenth, 15(28), 1-12.
Takarada, Y., Sato, Y., & Ishii, N. (2002). Effects of resistance exercise combined with vascular occlusion on muscle function in athletes. European Journal of Applied Physiology, 86, 308–14.
Terblanche, S. E. (1989). Recent advances in hormonal response to exercise. Comparative Biochemistry and Physiology - Biochemistry, 93(4), 727-39.
Woods, S. C., Benoit, S. C., Clegg, D. J., & Seeley, R. J. (2004). Regulation of energy homeostasis by peripheral signals. Best Practice & Research Clinical Endocrinology & Metabolism, 18(4), 497-515.
Yasuda, T., Abe, T., Sato, Y., Midorikawa, T., CF, K., Inoue, K., ... & Ishii, N. (2005). Muscle fiber cross-sectional area is increased after two weeks of twice daily KAATSU-resistance training. International Journal of KAATSU Training Research, 1(2), 65-70.