Document Type : Original Article

Authors

1 Ph.D in Exercise Physiology, Faculty of Sport Sciences, Razi University, Kermanshah, Iran

2 Associate Professor of Biological Sciences Department, Faculty of Sport Sciences, Razi University, Kermanshah, Iran

3 Full Professor in Medical Physiology, Department of Medical, Tabriz University of Medical Sciences,Tabriz, Iran

Abstract

Background and Aim: Inactivity and imbalance diet increase the risk of metabolic syndrome, diabetes, and cardiovascular diseases. Regarding the effects of training and garlic extract consumption on glucose, insulin, and lipid profile; this study was conducted to investigate the effects of 8-weeks of endurance and resistance training, and garlic extract supplementation on Bax gene expression, insulin resistance, and nitric oxide (NO) in rats with metabolic syndrome. Materials and Methods: A total of 48 rats with metabolic syndrome (aged 12-wk, mean weight of 325.36±25.22 g) were randomly divided into 6 groups (N=6) as: endurance training (ET), resistance training (RT), garlic extract supplementation (GE), endurance training along with garlic extract supplementation (ET+GE), resistance training along with garlic extract supplementation (RT+GE), and control (con). After familiarization, rats in the experimental groups completed an 8-weeks (3 sessions a week) ET or RT exercise protocol with or without GE (500 mg.kg-1.day-1) supplementation. The Rt-PCR and the Elisa methods were used to assess the Bax gene expression and the serum levels variables, respectively. Data were analysed using one-way ANOVA and the Tukey test (p˂0.05). Results: The results showed that ET and RT with and without the GE consumption significantly reduced the Bax Gene expression (p=0.01) and the insulin resistance (p=0.01) but for the nitric oxide levels (p=0.01) it showed significantly increased. Moreover, increase of the nitric oxide levels in the GE group was significantly greater than the RT+GE, ET and RT groups (p=0.0001). Conclusion: Endurance and resistance training with GE consumption have superior effects on the nitric oxide, insulin, and glucose in rats and can decrease the expression of cardiac apoptosis marker (Bax gene).

Keywords

Amaral, F., Lima, N. E., Ornelas, E., Simardi, L., Fonseca, F. L., & Maifrino, L. B. (2015). Effect of different exercise intensities on the pancreas of animals with metabolic syndrome. Diabetes Metabolic Syndrome Obesity: Targets and Therapy, 13(8), 115-20.
Augusti, K.T. (1996). Therapeutic values of onion (Allium cepa) and Garlic (Allium sativum). Indian Journal of Experimental Biology, 34(7), 634-40.
Bakri, I. M., & Douglas, C.W. (2005). Inhibitory effect of garlic extract on oral bacteria. Archives of Oral Biology, 50(7), 645-51.
Brüne, B., Zhou, J., & Von Knethen, A. (2003). Nitric oxide, oxidative stress, and apoptosis. Kidney International Suppllement, 63(84), S22-4.
Chavoshan, B., Fournier, M., Lewis, M. I., Porszasz, J., Storer, T. W., Da, X., … & Casaburi, R. (2012). Testosterone and resistance training effects on muscle nitric oxide synthase isoforms in COPD men. Respiratory Medicine,106(2), 269-75.
Conceição, M. S., Bonganha, V., Vechin, F. C., de Barros Berton, R. P., Lixandrão, M. E., Nogueira, F. R. D.,... & Libardi, C. A. (2013). Sixteen weeks of resistance training can decrease the risk of metabolic syndrome in healthy postmenopausal women. Clinical Interventions in Aging, 8, 1221.
Cornelissen, V. A., Goetschalckx, K., Verheyden, B., Aubert, A. E., Arnout, J., Persu, A., & Fagard, R. H. (2011). Effect of endurance training on blood pressure regulation, biomarkers and the heart in subjects at a higher age. Scandinavian Journal of Medicine & Science in Sports, 21(4),526-34.
Cheng, S. M., Cheng, Y. J., Wu, L. Y., Kuo, C. H., Lee, Y. S., Wu, M. C., & Lee, S. D. (2014). Activated apoptotic and anti-survival effects on rat hearts with fructose induced metabolic syndrome. Cell Biochemistry and Function, 32(2),133-41.
Drouin, R., Robert, G., Milot, M., Massicotte, D., Péronnet, F., &  Lavoie, C. (2004). Swim training increases glucose output from liver perfused in situ with glucagon in fed and fasted rats. Metabolism, 53(8),1027-31.
Durand, M.J., & Gutterman, D.D. (2014). Exercise and vascular function: how much is too much? Canadian journal of physiology and pharmacology,92(7), 551-7.
Elkayam, A., Mirelman, D., Peleg, E., Wilchek, M., Miron, T., Rabinkov, A., ... & Rosenthal, T. (2003). The effects of allicin on weight in fructose-induced hyperinsulinemic, hyperlipidemic, hypertensive rats. American Journal of Hypertension, 16(12), 1053-1056.
Elkayam, A., Peleg, E., Grossman, E., Shabtay, Z., & Sharabi, Y. (2013). Effects of allicin on cardiovascular risk factors in spontaneously hypertensive rats. Medical Association Journal, 15(3),170-3.
Eringa, E. C., Stehouwer, C. D., van Nieuw Amerongen, G. P., Ouwehand, L., Westerhof, N., & Sipkema, P. (2004). Vasoconstrictor effects of insulin in skeletal muscle arterioles are mediated by ERK1/2 activation in endothelium. American Journal of Physiology-Heart and Circulatory Physiology,287(5), 2043-8.
Esfahani, P. S., Gharakhanlou, R., Karimian, J., Khazaei, M., Feizi, A., & Safarzade, A. (2013).Effect of resistance training on plasma nitric oxide and asymmetric dimethylarginine concentrations in type I diabetic rats. International Journal of Preventive Medicine, 4(1), S78–S84.
Ghezzi, A. C., Cambri, L.T., Botezelli, J. D., Ribeiro, C., Dalia, R. A., & de Mello, M. A. R. (2012). Metabolic syndrome markers in wistar rats of different ages. Diabetology & Metabolic Syndrome, 4(1),16.
Granger, D. L., Taintor, R. R., Boockvar, K. S., & Hibbs Jr, J. B. (1996).  Measurement of nitrate and nitrite in biological samples using nitrate reductase and griess reaction. Methods in Enzymology, 268, 142-51.
Hariri, N., & Thibault, L. (2010). High-fat diet-induced obesity in animal models. Nutrition Research Reviews, 23(2), 270-99.
Heo, M., & Kim, E. (2013). Effects of endurance training on lipid metabolism and glycosylated hemoglobin levels in streptozotocin-induced type 2 diabetic rats on a high-fat diet. Journal of Physical Therapy Science, 25(8), 989-92.
Hrebícek, J., Janout, V., Malincíková, J., Horáková, D., & Cízek, L. (2002). Detection of insulin resistance by simple quantitative insulin sensitivity check index QUICKI for epidemiological assessment and prevention. The Journal of Clinical Endocrinology & Metabolism, 87(1), 144-7.
Hu, G., Qiao, Q., Tuomilehto, J., Balkau, B., Borch-Johnsen, K., & Pyorala, K. (2004). Prevalence of the metabolic syndrome and its relation to all-cause and cardiovascular mortality in nondiabetic European men and women. Archives of Internal Medicine, 164(10), 1066-76. 
Huang, C. Y., Lin, Y. Y., Hsu, C. C., Cheng, S. M., Shyu, W. C., Ting, H., ... & Lee, S. D. (2016). Antiapoptotic effect of exercise training on ovariectomized rat hearts. Journal of Applied Physiology, 121(2), 457-465.
Karimi, H., Rehman, S. S. U., & Gillani, S. A. (2017). Effects of supervised structured aerobic exercise training program on interleukin-6, nitric oxide synthase-1, and cyclooxygenase-2 in type 2 diabetes mellitus. Journal of the College of Physicians & Surgeons Pakistan,27(6), 352-355.
Karimian, J., Khazaei, M., & Shekarchizadeh, P. (2015). Effect of resistance training on capillary density around slow and fast twitch muscle fibers in diabetic and normal rats. Asian Journal of Sports Medicine,6(4), e24040.
Krause, M., Rodrigues-Krause, J., O'Hagan, C., Medlow, P., Davison, G., Susta, D., … & De Vito, G. (2014). The effects of aerobic exercise training at two different intensities in obesity and type 2 diabetes: implications for oxidative stress, low-grade inflammation and nitric oxide production. European Journal of Applied Physiology, 114(2), 251-260.
Kwak, H. B., Song, W., & Lawler, J. M. (2006). Exercise training attenuates age-induced elevation in Bax/Bcl-2 ratio, apoptosis, and remodeling in the rat heart. Federation of American Societies for Experimental Biology Journal, 20(6), 791-3.
Lee, M. O. (1929). Determination of the surface area of the white rat with it's application to the expression of metabolic results. American Journal of Physiology-Legacy Content, 89(1), 24-33.
Lin, Y. H., Chu, L. L., Kao, C. C., Chen, T. B., Lee, I., & Li, H. C. (2015). The effects of a diet and exercise program for older adults with metabolic syndrome. Journal of Nursing Research, 23(3), 197-205.
Morihara, N., Sumioka, I., Moriguchi, T., Uda, N., & Kyo, E. (2002). Aged garlic extract enhances production of nitric oxide. Life Sciences, 71(5),509-17.
Nassis, G. P., Papantakou, K., Skenderi, K., Triandafillopoulou, M., Kavouras, S. A., Yannakoulia, M., ... & Sidossis, L. S. (2005). Aerobic exercise training improves insulin sensitivity without changes in body weight, body fat, adiponectin, and inflammatory markers in overweight and obese girls. Metabolism, 54(11), 1472-1479.
Padiya, R., Khatua, T. N., Bagul, P. K., Kuncha, M., & Banerjee, S. K. (2011). Garlic improves insulin sensitivity and associated metabolic syndromes in fructose fed rats.  Nutrition & Metabolism, 8(1), 53.
Paneni, F., Costantino, S., & Cosentino, F. (2015). Role of oxidative stress in endothelial insulin resistance. World Journal of Diabetes, 6(2),326-32.
Pang, J., Xi, C., Huang, X., Cui, J., Gong, H., & Zhang, T. (2016). Effects of excess energy intake on glucose and lipid metabolism in C57BL/6 mice.  Public Library of Science One, 11(1),208-13.
PerezPimiento, A. J., Santaolalla, M., De Paz, S., Fernándezparra, B., Domínguezlázaro, A. R., & Moneo, I. (1999). Anaphylactic reaction to young garlic. Allergy, 54(6), 626-629.
Podestà, F., Romeo, G., Liu, W.H., Krajewski, S., Reed, J.C., Gerhardinger, C.,& Lorenzi, M. (2000). Bax is increased in the retina of diabetic subjects and is associated with pericyte apoptosis in vivo and in vitro.TheAmerican Journal of Pathology, 156(3), 1025-32.
Reaven, G. M. (1997). Banting lecture 1988: role of insulin resistance in human disease. Nutrition, 13(1), 64.
Sansbury, B. E., & Hill, B. G. (2014). Regulation of obesity and insulin resistance by nitric oxide. Free Radical Biology and Medicine, 73, 383-99.
Savas, M., Yeni, E., Ciftci, H., Yildiz, F., Gulum, M., Keser, B. S., ... & Bitiren, M. (2010). The antioxidant role of oral administration of garlic oil on renal ischemia–reperfusion injury. Renal failure, 32(3), 362-367.
Shirai, T., Shichi, Y., Sato, M., Tanioka, Y., Furusho, T., Ota, T., ... & Yamamoto, Y. (2016). High dietary fat–induced obesity in Wistar rats and type 2 diabetes in nonobese Goto-Kakizaki rats differentially affect retinol binding protein 4 expression and vitamin A metabolism. Nutrition Research, 36(3), 262-270.
Sung, R. Y., Tong, P. C., Yu, C. W., Lau, P. W., Mok, G. T., Yam, M. C., ... & Chan, J. C. (2003). High prevalence of insulin resistance and metabolic syndrome in overweight/obese preadolescent Hong Kong Chinese children aged 9–12 years. Diabetes Care, 26(1), 250-251.
Takashima, M., Kanamori, Y., Kodera, Y., Morihara, N., & Tamura, K. (2017). Aged garlic extract exerts endothelium-dependent vasorelaxant effect on rat aorta by increasing nitric oxide production. Phytomedicine, 24, 56-61.
Tibana, R. A., Navalta, J., Bottaro, M., Vieira, D., Tajra, V., de Oliveira Silva, A., ... & Cavaglieri, C. R. (2013). Effects of eight weeks of resistance training on the risk factors of metabolic syndrome in overweight/obese women-“A Pilot Study”. Diabetology & metabolic syndrome, 5(1), 11.
van der Heijden, G. J., Wang, Z. J., Chu, Z. D., Sauer, P. J., Haymond, M. W., Rodriguez, L. M., & Sunehag, A. L. (2010). A 12week aerobic exercise program reduces hepatic fat accumulation and insulin resistance in obese, Hispanic adolescents. Obesity, 18(2), 384-390.
Zhang, Y., Yao, H. P., Huang, F. F., Wu, W., Gao, Y., Chen, Z. B., ... & Liang, T. B. (2008). Allicin, a major component of garlic, inhibits apoptosis in vital organs in rats with trauma/hemorrhagic shock. Critical care medicine, 36(12), 3226-3232.