University of Birjand Journal of Practical Studies of Biosciences in Sport 2383-0182 12 32 2024 12 21 The effect of swimming training on hypocampic mir-23b and mir-let-7 gene expression related to pain in Wistar rats with Parkinson’s disease The effect of swimming training on hypocampic mir-23b and mir-let-7 gene expression related to pain in Wistar rats with Parkinson’s disease 20 29 2762 10.22077/jpsbs.2024.7032.1840 FA Reza Zeinolebadi PhD student in exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran 0009-0009-4472-9964 Mehrzad Moghadasi Associate professor of exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran Mohammdamin Edalatmanesh Associate Professor at Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran. Mehdi Noora Assistant professor of exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran Journal Article 2023 12 10 <strong>Background and Aim</strong>: Dysregulated miRNAs play critical roles in many disorders such as pain in Parkinson’s disease (PD). The aim of this study was to examine the effect of swimming training on hypocampic miRNAs gene expression related to pain in rats with PD. <strong>Materials and Methods</strong>: Twenty-one male Wistar rats (age eight to 10 weeks) were divided (n=7) into healthy control, PD, and training groups. PD was induced by injection of one mg/kg Reserpine to rats in PD and training groups. The rats in the training group performed six weeks of high-intensity interval training, including 20 times of 30 seconds of swimming with 30 seconds rest between each time and three times a week. mir-23b and mir-let-7 gene expressions were measured in hippocampus using Real Time-PCR method. Data were analyzed using one-way ANOVA and LSD tests at a significant level of p<0.05. <strong>Results</strong>: The  hypocampic mir-23b gene expression was significantly lower in PD compare to the healthy control group (p=0.01), while no significant difference was observed between PD and training groups (p=0.22); and between healthy control and training groups (p=0.09). On the other hand, hypocampic mir-let-7 gene expression did not significantly difference between PD  and healthy control group (p=0.50); moreover, no significant difference was observed between PD and training (p=0.82); and healthy and training (p=0.64) groups. <strong>Conclusion</strong>: It seems that high-intensity interval swimming training did not affect miRNAs related pain in rats with PD; however, due to existing limitations, more investigations are needed. <strong>Background and Aim</strong>: Dysregulated miRNAs play critical roles in many disorders such as pain in Parkinson’s disease (PD). The aim of this study was to examine the effect of swimming training on hypocampic miRNAs gene expression related to pain in rats with PD. <strong>Materials and Methods</strong>: Twenty-one male Wistar rats (age eight to 10 weeks) were divided (n=7) into healthy control, PD, and training groups. PD was induced by injection of one mg/kg Reserpine to rats in PD and training groups. The rats in the training group performed six weeks of high-intensity interval training, including 20 times of 30 seconds of swimming with 30 seconds rest between each time and three times a week. mir-23b and mir-let-7 gene expressions were measured in hippocampus using Real Time-PCR method. Data were analyzed using one-way ANOVA and LSD tests at a significant level of p<0.05. <strong>Results</strong>: The  hypocampic mir-23b gene expression was significantly lower in PD compare to the healthy control group (p=0.01), while no significant difference was observed between PD and training groups (p=0.22); and between healthy control and training groups (p=0.09). On the other hand, hypocampic mir-let-7 gene expression did not significantly difference between PD  and healthy control group (p=0.50); moreover, no significant difference was observed between PD and training (p=0.82); and healthy and training (p=0.64) groups. <strong>Conclusion</strong>: It seems that high-intensity interval swimming training did not affect miRNAs related pain in rats with PD; however, due to existing limitations, more investigations are needed. https://jpsbs.birjand.ac.ir/article_2762_8a502978a849f2c0760d5948093edfbd.pdf