نقش تمرین هوازی در بیان پروتئین عامل مشتق از سلول استرومال آلفا و مرگ آپوپتوزی سلول‌های قلبی پس از انفارکتوس تجربی میوکارد

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران.

2 استادیار، گروه فیزیولوژی ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه ایلام، ایلام، ایران

3 دانشیار، گروه پاتولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران.

چکیده

زمینه و هدف: انفارکتوس میوکارد یکی از دلایل اصلی مرگ و میر در سرتاسر جهان است که به وسیله­ی نکروز ایسکمیک ایجاد می ­شود. هدف تحقیق حاضر بررسی نقش تمرین هوازی در بیان پروتئین SDF-1/CXCR4 و آپوپتوز سلول­های قلبی به دنبال انفارکتوس تجربی میوکارد در موش­های صحرایی نر بود. روش تحقیق: 26 سر موش صحرایی نر (وزن 5 ± 235 گرم) به 4 گروه سالم (شم)، کنترل، تمرین هوازی و پایه تقسیم شدند. جهت القاء انفارکتوس میوکارد از تزریق زیرجلدی ایزوپرنالین (mg/kg 150) در دو روز متوالی بلافاصله 24 ساعت استفاده شد. گروه تمرین هوازی، 48 ساعت پس از القاء انفارکتوس میوکارد برنامه­ تمرینی را شروع کردند. برنامه تمرین شامل 4 هفته، 5 جلسه در هفته دویدن بر روی نوارگردان با سرعت 16-10 متر در دقیقه و مدت 50-10 دقیقه با شیب 5 درجه بود. جهت بررسی بیان پروتئین و آپوپتوز به ترتیب از رنگ ­آمیزی ایمونوهیستوشیمی (IHC) و رنگ­آمیزی تانل استفاده شد. داده ­ها با استفاده از آزمون آنالیز واریانس یک طرفه در سطح 05/0>p تحلیل شدند. یافته ­ها: نتایج نشان داد که تزریق زیرجلدی ایزوپرنالین باعث القاء آپوپتوز و افزایش بیان پروتئین عامل مشتق از سلول استرومال آلفا و گیرنده­ آن در بافت قلب موش های انفارکتوسی گردید. همچنین، نتایج نشان داد که بین گروه تمرین هوازی و گروه کنترل تفاوت معنی­ داری (001/0p<) از نظر تعداد سلول­های آپوپتوزی وجود داشت، اما از نظر میزان تظاهر پروتئین SDF-1/CXCR4 بین دو گروه فوق تفاوت آماری معنی ­داری مشاهده نگردید(05/0<p). نتیجه­ گیری: به طور کلی نتایج تحقیق نشان داد که انجام تمرین هوازی بعد از انفارکتوس میوکارد می­ تواند باعث کاهش مرگ آپوپتوزی سلول­ های قلبی گردد، اما تأثیر معنی ­داری بر تظاهر پروتئین مسیرهای سیگنالینگ مؤثر در رگ­زایی مانند SDF-1/CXCR4 ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

The role of aerobic training in protein expression of stromal cell-derived factor-alpha and apoptotic death of cardiomyocyte after experimental myocardial infarction

نویسندگان [English]

  • Hadi Abdi 1
  • Nabi Shamsaei 2
  • Mohammad Jafari 3
1 Assistant Professor, Department of Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.
2 Assistant Professor of Exercise Physiology Department, Faculty of Faculty of Literature and Humanities, Ilam University, Ilam, Iran.
3 Associate Professor of Pathology Department, Faculty of Medicine, Hamadan University of Medical Sciences, Hamedan, Iran.
چکیده [English]

Background and Aim: Purpose of this study was to investigate the role of aerobic training in protein expression of SDF-1/CXCR4 and cardiomyocyte apoptosis following experimental myocardial infarction in rats. Materials and Methods: Twenty six male rats (weight 235 ± 5) were divided into healthy (sham), control, aerobic training and basic group. To induce myocardial infarction subcutaneous injection of isoprenaline (150 mg/kg) on two consecutive days with an interval of 24 hours was used. Aerobic training group started training program 48 hours after induction of myocardial infarction. Training  program include 4 weeks, 5 sessions a week running on a treadmill at a speed of 10-16 meters per minute and a period of 10-50 minutes with a slope of 5 degree. To study the protein expression and apoptosis, immunohistochemistry (IHC) and TUNNEL staining was used respectively. Data were analyzed using one-way ANOVA at pResults: The results showed that injection of isoprenaline induced apoptosis and increased protein expression of stromal cell-derived factor-alpha and its receptor in infarcted rat hearts. Also, results showed that there was significant difference (p<0.001) in the number of apoptotic cells between training and control group, but in terms of SDF-1/CXCR4 protein expression significant difference was not observed between two groups. Conclusion: Overall results showed that aerobic training after myocardial infarction can reduce the apoptosis of cardiomyocyte, but does not significant effect on protein expression of signaling pathways involved in angiogenesis such as SDF-1/CXCR4.

کلیدواژه‌ها [English]

  • Aerobic training
  • Stromal cell-derived factor-alpha
  • Apoptotic death
  • Experimental myocardial infarction

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