تاثیر تمرین استقامتی بر بیان miR-133 و ژنSRF عضلات اسکلتی تند و کند انقباض در رت های نر نژاد ویستار

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجو دکترای فیزیولوژی ورزش دانشگاه شهید چمران اهواز، اهواز، ایران

2 دانشیار گروه فیزیولوژی ورزش، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استادیار گروه ژنتیک مولکولی، دانشگاه لرستان، خرم آباد، ایران

چکیده

زمینه و هدف: عوامل  miR-133وSRF  در فرآیندهای مختلف سلولی درگیر می باشند، اما هنوز تأثیر تمرین استقامتی بر بیان آنها در عضلات تند و کند انقباض مشخص نشده است. هدف این تحقیق ارزیابی تاثیر تمرین استقامتی بر بیان miR-133 و SRF عضلات تند و کند انقباض در رت‌های نر نژاد ویستار بود. روش تحقیق: در این مطالعه 14 رت با وزن 20±113 گرم (5 هفته سن داشتند) به مدت 4 هفته تحت شرایط کنترل شده، نگهداری شدند و بعد از دوره آشناسازی به صورت تصادفی به دو گروه کنترل و تجربی (7 سر رت در هر گروه) تقسیم شدند. گروه تجربی یک برنامه تمرین استقامتی 14 هفته ای، هفته ای 6 جلسه (که بتدریج به 60 دقیقه و سرعت 30 متر بر دقیقه رسید) را روی نوارگردان اجرا نمودند و 48 ساعت پس از پایان آخرین جلسه، همراه با گروه کنترل تشریح شدند. سپس عضله کند انقباض (نعلی) و تند انقباض (بازکننده بلند انگشتان) آنها جدا شد. سطح بیان  miR-133و ژن SRF با استفاده از روش Real time-PCR اندازه گیری شدند. نتایج تحقیق با استفاده از آزمون آماری t تک نمونه و همبسته در سطح 0/01>p استخراج گردید. یافته ها: پس از 14 هفته تمرین استقامتی، بیان miR-133 عضله بازکننده بلند انگشتان گروه تجربی نسبت به گروه کنترل به طور معنی‌داری (0/001=p) کاهش پیدا کرد؛ اما بیان miR-133 عضله نعلی افزایش معنی‌داری (001/0=p) نشان داد. همچنین بیان  miR-133عضله بازکننده بلند انگشتان گروه تجربی نسبت به عضله نعلی همان گروه به طور معنی‌داری (0/001=p) کاهش و میزان بیان ژن SRF عضله بازکننده بلند انگشتان گروه تجربی به طور معنی داری (0/009=p) افزایش یافت.  اما بیان این ژن در عضله نعلی تغییر نکرد (0/05<p). نتیجه گیری: تمرین استقامتی باعث القاء تغییراتی در بیان miR-133  و ژن SRF متناسب با خصوصیات تارهای تند و کند انقباض می شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of endurance training on miR-133 expression and SRF gene in slow and fast twitch skeletal muscles of wistar rats

نویسندگان [English]

  • Raziyeh Rezaei 1
  • Saeed Shakeriyan 2
  • Masoud Nikbakht 2
  • Faranak Hadi 3
1 Ph.D Student of Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 Associate Professorof Exercise Physiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Assistant of Molecular Genetic, Lorestan university, Khoramabad, Iran
چکیده [English]

Background And Aim: MicroRNA-133 and SRF is involved in various cellular processes, but the effect of endurance training on gene expression of this factors in fast and slow twitch muscles has still remained unclear. The aim of this study was to evaluate the effect of endurance training on miR-133 and SRF gene expression in fast and slow twitch muscles in male Wistar rats. Materials and Methods: In this study, 14 rats weighing 113±20 grams (5 weeks age) were housed under controlled conditions, after familiarization protecols they were randomly assigned into control (7 rats) and experimental (7 rats) groups. The experimental group performed 14 weeks, 6 session per week endurance training program (that gradually reached to 60 min and 30 m / min) on treadmill and 48 hours after the end of the last session, both groups were sacrificed. The soleus and EDL muscles were removed. The gene expression level of miR-133 and SRF were measured using real- time RT-PCR. Data were analysd by sample t-test. Results: After 14 weeks of endurance training the gene expression of miR-133 of fast twitch muscle (EDL) in experimental group significantly decreased (p=0.001) than control group .But the miR-133 gene expression of slow twitch muscle (soleus) in experimental group was significantly increased (p=0.001) than control group. Also miR-133 gene expression of EDL of experimental group was significantly decresed (p=0.001) than soleus muscle of the same group; the rate of SRF gene expression of EDL of experimental group increased significantly (p=0.009) but it did not effect on SRF gene expression in soleus. Conclusion: Endurance training induced differences in gene expression of miR-133 and SRF gene consistent to specification of fast and slow twitch muscles.

کلیدواژه‌ها [English]

  • Endurance training
  • Fast twitch muscle
  • Slow twitch muscle
  • SRF gene
  • miR-133
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